Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium

Louise Kjærulff; Anita Nielsen; Maria Mansson; Lone Gram; Thomas Ostenfeld Larsen; Hanne Ingmer; Charlotte Held Gotfredsen

doi:10.3390/md11125051

Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium

Louise Kjærulff, Anita Nielsen, Maria Mansson, Lone Gram, Thomas Ostenfeld Larsen, Hanne Ingmer, Charlotte Held Gotfredsen

Food Safety and Zoonoses

33 Citations (Scopus)

Abstract

During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500-900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.

Original language	English
Journal	Marine Drugs
Volume	11
Issue number	12
Pages (from-to)	5051-5062
Number of pages	12
ISSN	1660-3397
DOIs	https://doi.org/10.3390/md11125051
Publication status	Published - Dec 2013

Keywords

Faculty of Health and Medical Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/md11125051

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title = "Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium",

abstract = "During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500-900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.",

keywords = "Faculty of Health and Medical Sciences, Photobacterium, depsipeptide, structure elucidation, quorum sensing, antivirulence, agr",

author = "Louise Kj{\ae}rulff and Anita Nielsen and Maria Mansson and Lone Gram and Larsen, {Thomas Ostenfeld} and Hanne Ingmer and Gotfredsen, {Charlotte Held}",

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doi = "10.3390/md11125051",

language = "English",

volume = "11",

pages = "5051--5062",

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T1 - Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium

AU - Kjærulff, Louise

AU - Nielsen, Anita

AU - Mansson, Maria

AU - Gram, Lone

AU - Larsen, Thomas Ostenfeld

AU - Ingmer, Hanne

AU - Gotfredsen, Charlotte Held

PY - 2013/12

Y1 - 2013/12

N2 - During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500-900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.

AB - During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500-900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.

KW - Faculty of Health and Medical Sciences

KW - Photobacterium

KW - depsipeptide

KW - structure elucidation

KW - quorum sensing

KW - antivirulence

KW - agr

U2 - 10.3390/md11125051

DO - 10.3390/md11125051

M3 - Journal article

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SN - 1660-3397

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SP - 5051

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JO - Marine Drugs

JF - Marine Drugs

IS - 12

ER -

Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium

Abstract

Keywords

UN SDGs

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