Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium

TY - JOUR

T1 - Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium

AU - Kjærulff, Louise

AU - Nielsen, Anita

AU - Mansson, Maria

AU - Gram, Lone

AU - Larsen, Thomas Ostenfeld

AU - Ingmer, Hanne

AU - Gotfredsen, Charlotte Held

PY - 2013/12

Y1 - 2013/12

N2 - During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500-900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.

AB - During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500-900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.

KW - Faculty of Health and Medical Sciences

KW - Photobacterium

KW - depsipeptide

KW - structure elucidation

KW - quorum sensing

KW - antivirulence

KW - agr

U2 - 10.3390/md11125051

DO - 10.3390/md11125051

M3 - Journal article

C2 - 24351904

SN - 1660-3397

VL - 11

SP - 5051

EP - 5062

JO - Marine Drugs

JF - Marine Drugs

IS - 12

ER -