Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa.

TY - JOUR

T1 - Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa.

AU - Li, Yingli

AU - Xia, Huiming

AU - Bai, Fang

AU - Xu, Haijin

AU - Yang, Liang

AU - Yao, Hongming

AU - Zhang, Lu

AU - Zhang, Xiuming

AU - Bai, Yanling

AU - Saris, Per E J

AU - Tolker-Nielsen, Tim

AU - Qiao, Mingqiang

N1 - Keywords: Artificial Gene Fusion; Biofilms; Chemotaxis; DNA Transposable Elements; DNA, Bacterial; Flagella; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Reporter; Genetic Complementation Test; Locomotion; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Molecular Sequence Data; Mutagenesis, Insertional; Operon; Pseudomonas aeruginosa; Sequence Analysis, DNA; beta-Galactosidase

PY - 2007

Y1 - 2007

N2 - Flagella-mediated motility is recognized as one of the major factors contributing to virulence in Pseudomonas aeruginosa. During a screening of a mini-Mu transposon mutant library of P. aeruginosa PA68, a mutant partially deficient in swimming and swarming motility was identified in a new locus that encodes a predicted protein of unknown function annotated PA5017 in the P. aeruginosa PAO1 genome sequence. Chemotaxis plate assay indicated that inactivation of the PA5017 gene led to a decreased chemotactic response. Complementation of the PA5017 mutant with the wild-type PA5017 gene restored normal motility and chemotaxis phenotype. A promoter-lacZ reporter activity assay of the cheYZAB operon from chemotaxis gene cluster 1 showed that there was almost a twofold difference in expression levels of the wild-type PA68 and the PA5017 mutant. This suggested that the PA5017 affected expression of the cheYZAB operon negatively. Further study showed that inactivation of the PA5017 gene in PA68 led to increased biofilm formation in a static system and to the formation of a heterogeneous biofilm in a flow-chamber system. These results suggested that PA5017 possibly affected flagellum-dependent motility and in turn biofilm formation via the chemotaxis signal transduction pathway.

AB - Flagella-mediated motility is recognized as one of the major factors contributing to virulence in Pseudomonas aeruginosa. During a screening of a mini-Mu transposon mutant library of P. aeruginosa PA68, a mutant partially deficient in swimming and swarming motility was identified in a new locus that encodes a predicted protein of unknown function annotated PA5017 in the P. aeruginosa PAO1 genome sequence. Chemotaxis plate assay indicated that inactivation of the PA5017 gene led to a decreased chemotactic response. Complementation of the PA5017 mutant with the wild-type PA5017 gene restored normal motility and chemotaxis phenotype. A promoter-lacZ reporter activity assay of the cheYZAB operon from chemotaxis gene cluster 1 showed that there was almost a twofold difference in expression levels of the wild-type PA68 and the PA5017 mutant. This suggested that the PA5017 affected expression of the cheYZAB operon negatively. Further study showed that inactivation of the PA5017 gene in PA68 led to increased biofilm formation in a static system and to the formation of a heterogeneous biofilm in a flow-chamber system. These results suggested that PA5017 possibly affected flagellum-dependent motility and in turn biofilm formation via the chemotaxis signal transduction pathway.

U2 - 10.1111/j.1574-6968.2007.00753.x

DO - 10.1111/j.1574-6968.2007.00753.x

M3 - Journal article

C2 - 17521365

SN - 0378-1097

VL - 272

SP - 188

EP - 195

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

IS - 2

ER -