Identification and expression profiling of low oxygen regulated genes from Citrus flavedo tissues using RT-PCR differential display

TY - JOUR

T1 - Identification and expression profiling of low oxygen regulated genes from Citrus flavedo tissues using RT-PCR differential display

AU - Pasentsis, Konstantinos

AU - Falara, Vasiliki

AU - Pateraki, Irene

AU - Gerasopoulos, Dimitrios

AU - Kanellis, Angelos K.

AU - Pateraki, Eirini

PY - 2007/4/1

Y1 - 2007/4/1

N2 - The molecular basis for the adaptation of fruit tissues to low oxygen treatments remains largely unknown. RT-PCR differential display (DD) was employed to isolate anoxic and/or hypoxic genes whose expression responded to short, low-oxygen regimes. This approach led to the isolation, cloning, successful sequencing, and bioinformatic analysis of 98 transcripts from Citrus flavedo tissues that were differentially expressed in DD gels in response to 0, 0.5, 3, and 21% O2 for 24 h. RNA blot analysis of 25 DD clones revealed that 11 genes were induced under hypoxia and/or anoxia, 11 exhibited constitutive expression and three transcripts were suppressed by low oxygen levels. Almost half of the DD cDNAs were either of unknown function or shared no apparent homology to any expressed sequences in the GenBank/EMBL databases. Six DD genes were similar to molecules of the following functions: C-compound and carbohydrate utilization, plant development, amino acid metabolism, and biosynthesis of brasinosteroids. Time-course and stress-related experiments of low O2-regulated genes indicated that these genes responded differently in terms of their earliness, band intensity, and their specificity to stresses, showing that some of them can be termed hypoxia- or anoxia-induced genes.

AB - The molecular basis for the adaptation of fruit tissues to low oxygen treatments remains largely unknown. RT-PCR differential display (DD) was employed to isolate anoxic and/or hypoxic genes whose expression responded to short, low-oxygen regimes. This approach led to the isolation, cloning, successful sequencing, and bioinformatic analysis of 98 transcripts from Citrus flavedo tissues that were differentially expressed in DD gels in response to 0, 0.5, 3, and 21% O2 for 24 h. RNA blot analysis of 25 DD clones revealed that 11 genes were induced under hypoxia and/or anoxia, 11 exhibited constitutive expression and three transcripts were suppressed by low oxygen levels. Almost half of the DD cDNAs were either of unknown function or shared no apparent homology to any expressed sequences in the GenBank/EMBL databases. Six DD genes were similar to molecules of the following functions: C-compound and carbohydrate utilization, plant development, amino acid metabolism, and biosynthesis of brasinosteroids. Time-course and stress-related experiments of low O2-regulated genes indicated that these genes responded differently in terms of their earliness, band intensity, and their specificity to stresses, showing that some of them can be termed hypoxia- or anoxia-induced genes.

KW - Anoxia

KW - Citrus

KW - Citrus sinensis L.

KW - Flavedo

KW - Gene expression

KW - Hypoxia

KW - mRNA differential display

KW - Orange fruit

KW - Stress

U2 - 10.1093/jxb/erm078

DO - 10.1093/jxb/erm078

M3 - Journal article

C2 - 17525081

AN - SCOPUS:34547659280

SN - 0022-0957

VL - 58

SP - 2203

EP - 2216

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 8

ER -