TY - JOUR
T1 - Identification and expression profiling of low oxygen regulated genes from Citrus flavedo tissues using RT-PCR differential display
AU - Pasentsis, Konstantinos
AU - Falara, Vasiliki
AU - Pateraki, Irene
AU - Gerasopoulos, Dimitrios
AU - Kanellis, Angelos K.
AU - Pateraki, Eirini
PY - 2007/4/1
Y1 - 2007/4/1
N2 - The molecular basis for the adaptation of fruit tissues to low oxygen treatments remains largely unknown. RT-PCR differential display (DD) was employed to isolate anoxic and/or hypoxic genes whose expression responded to short, low-oxygen regimes. This approach led to the isolation, cloning, successful sequencing, and bioinformatic analysis of 98 transcripts from Citrus flavedo tissues that were differentially expressed in DD gels in response to 0, 0.5, 3, and 21% O2 for 24 h. RNA blot analysis of 25 DD clones revealed that 11 genes were induced under hypoxia and/or anoxia, 11 exhibited constitutive expression and three transcripts were suppressed by low oxygen levels. Almost half of the DD cDNAs were either of unknown function or shared no apparent homology to any expressed sequences in the GenBank/EMBL databases. Six DD genes were similar to molecules of the following functions: C-compound and carbohydrate utilization, plant development, amino acid metabolism, and biosynthesis of brasinosteroids. Time-course and stress-related experiments of low O2-regulated genes indicated that these genes responded differently in terms of their earliness, band intensity, and their specificity to stresses, showing that some of them can be termed hypoxia- or anoxia-induced genes.
AB - The molecular basis for the adaptation of fruit tissues to low oxygen treatments remains largely unknown. RT-PCR differential display (DD) was employed to isolate anoxic and/or hypoxic genes whose expression responded to short, low-oxygen regimes. This approach led to the isolation, cloning, successful sequencing, and bioinformatic analysis of 98 transcripts from Citrus flavedo tissues that were differentially expressed in DD gels in response to 0, 0.5, 3, and 21% O2 for 24 h. RNA blot analysis of 25 DD clones revealed that 11 genes were induced under hypoxia and/or anoxia, 11 exhibited constitutive expression and three transcripts were suppressed by low oxygen levels. Almost half of the DD cDNAs were either of unknown function or shared no apparent homology to any expressed sequences in the GenBank/EMBL databases. Six DD genes were similar to molecules of the following functions: C-compound and carbohydrate utilization, plant development, amino acid metabolism, and biosynthesis of brasinosteroids. Time-course and stress-related experiments of low O2-regulated genes indicated that these genes responded differently in terms of their earliness, band intensity, and their specificity to stresses, showing that some of them can be termed hypoxia- or anoxia-induced genes.
KW - Anoxia
KW - Citrus
KW - Citrus sinensis L.
KW - Flavedo
KW - Gene expression
KW - Hypoxia
KW - mRNA differential display
KW - Orange fruit
KW - Stress
U2 - 10.1093/jxb/erm078
DO - 10.1093/jxb/erm078
M3 - Journal article
C2 - 17525081
AN - SCOPUS:34547659280
SN - 0022-0957
VL - 58
SP - 2203
EP - 2216
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 8
ER -