Hyperphosphorylation of cereal starch

Massimiliano Carciofi; Shahnoor Sultana Shaik; Susanne Langgård Jensen; Per Gunnar Andreas Blennow; Jan Tommy Svensson; Eva Vincze; Kim Henrik Hebelstrup

doi:10.1016/j.jcs.2011.06.013

Hyperphosphorylation of cereal starch

Massimiliano Carciofi, Shahnoor Sultana Shaik, Susanne Langgård Jensen, Per Gunnar Andreas Blennow, Jan Tommy Svensson, Eva Vincze, Kim Henrik Hebelstrup

44 Citations (Scopus)

Abstract

Plant starch is naturally phosphorylated at a fraction of the C6 and the C3 hydroxyl groups during its biosynthesis in plastids. Starch phosphate esters are important in starch metabolism and they also generate specific industrial functionality. Cereal grains starch contains little starch bound phosphate compared with potato tuber starch and in order to investigate the effect of increased endosperm starch phosphate, the potato starch phosphorylating enzyme glucan water dikinase (StGWD) was overexpressed specifically in the developing barley endosperm. StGWD overexpressors showed wild-type phenotype. Transgenic cereal grains synthesized starch with higher starch bound phosphate content (7.5 (±0.67). nmol/mg) compared to control lines (0.8 (±0.05). nmol/mg) with starch granules showing altered morphology and lower melting enthalpy. Our data indicate specific action of GWD during starch biosynthesis and demonstrates the possibility for in planta production of highly phosphorylated cereal starch.

Original language	English
Journal	Journal of Cereal Science
Volume	54
Issue number	3
Pages (from-to)	339-346
Number of pages	8
ISSN	0733-5210
DOIs	https://doi.org/10.1016/j.jcs.2011.06.013
Publication status	Published - Nov 2011

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title = "Hyperphosphorylation of cereal starch",

abstract = "Plant starch is naturally phosphorylated at a fraction of the C6 and the C3 hydroxyl groups during its biosynthesis in plastids. Starch phosphate esters are important in starch metabolism and they also generate specific industrial functionality. Cereal grains starch contains little starch bound phosphate compared with potato tuber starch and in order to investigate the effect of increased endosperm starch phosphate, the potato starch phosphorylating enzyme glucan water dikinase (StGWD) was overexpressed specifically in the developing barley endosperm. StGWD overexpressors showed wild-type phenotype. Transgenic cereal grains synthesized starch with higher starch bound phosphate content (7.5 (±0.67). nmol/mg) compared to control lines (0.8 (±0.05). nmol/mg) with starch granules showing altered morphology and lower melting enthalpy. Our data indicate specific action of GWD during starch biosynthesis and demonstrates the possibility for in planta production of highly phosphorylated cereal starch.",

author = "Massimiliano Carciofi and Shaik, {Shahnoor Sultana} and Jensen, {Susanne Langg{\aa}rd} and Blennow, {Per Gunnar Andreas} and Svensson, {Jan Tommy} and Eva Vincze and Hebelstrup, {Kim Henrik}",

year = "2011",

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doi = "10.1016/j.jcs.2011.06.013",

language = "English",

volume = "54",

pages = "339--346",

journal = "Journal of Cereal Science",

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TY - JOUR

T1 - Hyperphosphorylation of cereal starch

AU - Carciofi, Massimiliano

AU - Shaik, Shahnoor Sultana

AU - Jensen, Susanne Langgård

AU - Blennow, Per Gunnar Andreas

AU - Svensson, Jan Tommy

AU - Vincze, Eva

AU - Hebelstrup, Kim Henrik

PY - 2011/11

Y1 - 2011/11

N2 - Plant starch is naturally phosphorylated at a fraction of the C6 and the C3 hydroxyl groups during its biosynthesis in plastids. Starch phosphate esters are important in starch metabolism and they also generate specific industrial functionality. Cereal grains starch contains little starch bound phosphate compared with potato tuber starch and in order to investigate the effect of increased endosperm starch phosphate, the potato starch phosphorylating enzyme glucan water dikinase (StGWD) was overexpressed specifically in the developing barley endosperm. StGWD overexpressors showed wild-type phenotype. Transgenic cereal grains synthesized starch with higher starch bound phosphate content (7.5 (±0.67). nmol/mg) compared to control lines (0.8 (±0.05). nmol/mg) with starch granules showing altered morphology and lower melting enthalpy. Our data indicate specific action of GWD during starch biosynthesis and demonstrates the possibility for in planta production of highly phosphorylated cereal starch.

AB - Plant starch is naturally phosphorylated at a fraction of the C6 and the C3 hydroxyl groups during its biosynthesis in plastids. Starch phosphate esters are important in starch metabolism and they also generate specific industrial functionality. Cereal grains starch contains little starch bound phosphate compared with potato tuber starch and in order to investigate the effect of increased endosperm starch phosphate, the potato starch phosphorylating enzyme glucan water dikinase (StGWD) was overexpressed specifically in the developing barley endosperm. StGWD overexpressors showed wild-type phenotype. Transgenic cereal grains synthesized starch with higher starch bound phosphate content (7.5 (±0.67). nmol/mg) compared to control lines (0.8 (±0.05). nmol/mg) with starch granules showing altered morphology and lower melting enthalpy. Our data indicate specific action of GWD during starch biosynthesis and demonstrates the possibility for in planta production of highly phosphorylated cereal starch.

U2 - 10.1016/j.jcs.2011.06.013

DO - 10.1016/j.jcs.2011.06.013

M3 - Journal article

SN - 0733-5210

VL - 54

SP - 339

EP - 346

JO - Journal of Cereal Science

JF - Journal of Cereal Science

IS - 3

ER -

Hyperphosphorylation of cereal starch

Abstract

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