TY - JOUR
T1 - Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings
AU - Selig, Michael Joseph
AU - Thygesen, Lisbeth Garbrecht
AU - Johnson, David K.
AU - E. Himmel, Michael
AU - Felby, Claus
AU - Mittal, Ashutosh
PY - 2013/10
Y1 - 2013/10
N2 - Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.
AB - Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.
U2 - 10.1007/s10529-013-1258-7
DO - 10.1007/s10529-013-1258-7
M3 - Journal article
C2 - 23881312
SN - 0141-5492
VL - 35
SP - 1599
EP - 1607
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 10
ER -