Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings

Michael Joseph Selig; Lisbeth Garbrecht Thygesen; David K.  Johnson; Michael   E. Himmel; Claus Felby; Ashutosh  Mittal

doi:10.1007/s10529-013-1258-7

Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings

Michael Joseph Selig, Lisbeth Garbrecht Thygesen, David K. Johnson, Michael E. Himmel, Claus Felby, Ashutosh Mittal

LUKKET: Skov, natur og biomasse

17 Citationer (Scopus)

Abstract

Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and III_I producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose III_I was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and III_I. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase III_I left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose III_I had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose III_I compared to celluloses Iβ and II.

Originalsprog	Engelsk
Tidsskrift	Biotechnology Letters
Vol/bind	35
Udgave nummer	10
Sider (fra-til)	1599–1607
Antal sider	9
ISSN	0141-5492
DOI	https://doi.org/10.1007/s10529-013-1258-7
Status	Udgivet - okt. 2013

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10.1007/s10529-013-1258-7

Citationsformater

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title = "Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings",

abstract = "Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.",

author = "Selig, {Michael Joseph} and Thygesen, {Lisbeth Garbrecht} and Johnson, {David K.} and {E. Himmel}, Michael and Claus Felby and Ashutosh Mittal",

year = "2013",

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doi = "10.1007/s10529-013-1258-7",

language = "English",

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pages = "1599–1607",

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T1 - Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings

AU - Selig, Michael Joseph

AU - Thygesen, Lisbeth Garbrecht

AU - Johnson, David K.

AU - E. Himmel, Michael

AU - Felby, Claus

AU - Mittal, Ashutosh

PY - 2013/10

Y1 - 2013/10

N2 - Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.

AB - Crystalline cellulose Iβ (Avicel) was chemically transformed into cellulose II and IIII producing allomorphs with similar crystallinity indices (ATR-IR and XRD derived). Saccharifications by commercial cellulases at arrayed solids loadings showed cellulose IIII was more readily hydrolysable and less susceptible to increased dry solids levels than cellulose Iβ and II. Analysis by dynamic vapor sorption revealed cellulose II has a distinctively higher absorptive capacity than cellulose I and IIII. When equally hydrated (g water/g cellulose), low-field nuclear magnetic resonance (LF-NMR) relaxometry showed that cellulose II, on average, most constrained water while cellulase IIII left the most free water. LF-NMR spin-spin relaxation time distribution profiles representing distinct water pools suggest cellulose IIII had the most restricted pool and changes in water distribution during enzymatic saccharification were most dramatic with respect to cellulose IIII compared to celluloses Iβ and II.

U2 - 10.1007/s10529-013-1258-7

DO - 10.1007/s10529-013-1258-7

M3 - Journal article

C2 - 23881312

SN - 0141-5492

VL - 35

SP - 1599

EP - 1607

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 10

ER -

Hydration and saccharification of cellulose Iβ, II and IIII at increasing dry solids loadings

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