Human anti-rhesus D IgG1 antibody produced in transgenic plants.

Thomas Bouquin; Mads Thomsen; Leif Kofoed Nielsen; Trine Hefsgaard Green; John Mundy; Morten Hanefeld Dziegiel

doi:10.1023/A:1015226418688

Human anti-rhesus D IgG1 antibody produced in transgenic plants.

Thomas Bouquin, Mads Thomsen, Leif Kofoed Nielsen, Trine Hefsgaard Green, John Mundy, Morten Hanefeld Dziegiel

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Abstract

Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

Original language	English
Journal	Transgenic Research
Volume	11
Issue number	2
Pages (from-to)	115-22
Number of pages	7
ISSN	0962-8819
DOIs	https://doi.org/10.1023/A:1015226418688
Publication status	Published - 2002

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title = "Human anti-rhesus D IgG1 antibody produced in transgenic plants.",

abstract = "Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.",

author = "Thomas Bouquin and Mads Thomsen and Nielsen, {Leif Kofoed} and Green, {Trine Hefsgaard} and John Mundy and Dziegiel, {Morten Hanefeld}",

note = "Keywords: Agglutination Tests; Arabidopsis; Blotting, Western; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; Humans; Immunoglobulin G; Monocytes; Plants, Genetically Modified; Recombinant Proteins; Respiratory Burst; Rh-Hr Blood-Group System",

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doi = "10.1023/A:1015226418688",

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T1 - Human anti-rhesus D IgG1 antibody produced in transgenic plants.

AU - Bouquin, Thomas

AU - Thomsen, Mads

AU - Nielsen, Leif Kofoed

AU - Green, Trine Hefsgaard

AU - Mundy, John

AU - Dziegiel, Morten Hanefeld

N1 - Keywords: Agglutination Tests; Arabidopsis; Blotting, Western; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; Humans; Immunoglobulin G; Monocytes; Plants, Genetically Modified; Recombinant Proteins; Respiratory Burst; Rh-Hr Blood-Group System

PY - 2002

Y1 - 2002

N2 - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

AB - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

U2 - 10.1023/A:1015226418688

DO - 10.1023/A:1015226418688

M3 - Journal article

C2 - 12054345

SN - 0962-8819

VL - 11

SP - 115

EP - 122

JO - Transgenic Research

JF - Transgenic Research

IS - 2

ER -

Human anti-rhesus D IgG1 antibody produced in transgenic plants.

Abstract

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