TY - JOUR
T1 - Human anti-rhesus D IgG1 antibody produced in transgenic plants.
AU - Bouquin, Thomas
AU - Thomsen, Mads
AU - Nielsen, Leif Kofoed
AU - Green, Trine Hefsgaard
AU - Mundy, John
AU - Dziegiel, Morten Hanefeld
N1 - Keywords: Agglutination Tests; Arabidopsis; Blotting, Western; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; Humans; Immunoglobulin G; Monocytes; Plants, Genetically Modified; Recombinant Proteins; Respiratory Burst; Rh-Hr Blood-Group System
PY - 2002
Y1 - 2002
N2 - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.
AB - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.
U2 - 10.1023/A:1015226418688
DO - 10.1023/A:1015226418688
M3 - Journal article
C2 - 12054345
SN - 0962-8819
VL - 11
SP - 115
EP - 122
JO - Transgenic Research
JF - Transgenic Research
IS - 2
ER -