How curved membranes recruit amphipathic helices and protein anchoring motifs

Nikos Hatzakis; Vikram Kjøller Bhatia; Jannik Larsen; Kenneth Lindegaard Madsen; Pierre-Yves Bolinger; Andreas Hjarne Kunding; John Jairo Castillo Leon; Ulrik Gether; Per Hedegård; Dimitrios Stamou

doi:10.1038/nchembio.213

How curved membranes recruit amphipathic helices and protein anchoring motifs

Nikos Hatzakis, Vikram Kjøller Bhatia, Jannik Larsen, Kenneth Lindegaard Madsen, Pierre-Yves Bolinger, Andreas Hjarne Kunding, John Jairo Castillo Leon, Ulrik Gether, Per Hedegård, Dimitrios Stamou

249 Citations (Scopus)

Abstract

Lipids and several specialized proteins are thought to be able to sense the curvature of membranes (MC). Here we used quantitative fluorescence microscopy to measure curvature-selective binding of amphipathic motifs on single liposomes 50-700 nm in diameter. Our results revealed that sensing is predominantly mediated by a higher density of binding sites on curved membranes instead of higher affinity. We proposed a model based on curvature-induced defects in lipid packing that related these findings to lipid sorting and accurately predicted the existence of a new ubiquitous class of curvature sensors: membrane-anchored proteins. The fact that unrelated structural motifs such as alpha-helices and alkyl chains sense MC led us to propose that MC sensing is a generic property of curved membranes rather than a property of the anchoring molecules. We therefore anticipate that MC will promote the redistribution of proteins that are anchored in membranes through other types of hydrophobic moieties.

Original language	English
Journal	Nature Chemical Biology
Volume	5
Issue number	11
Pages (from-to)	835-841
Number of pages	6
ISSN	1552-4450
DOIs	https://doi.org/10.1038/nchembio.213
Publication status	Published - 2009

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title = "How curved membranes recruit amphipathic helices and protein anchoring motifs",

abstract = "Lipids and several specialized proteins are thought to be able to sense the curvature of membranes (MC). Here we used quantitative fluorescence microscopy to measure curvature-selective binding of amphipathic motifs on single liposomes 50-700 nm in diameter. Our results revealed that sensing is predominantly mediated by a higher density of binding sites on curved membranes instead of higher affinity. We proposed a model based on curvature-induced defects in lipid packing that related these findings to lipid sorting and accurately predicted the existence of a new ubiquitous class of curvature sensors: membrane-anchored proteins. The fact that unrelated structural motifs such as alpha-helices and alkyl chains sense MC led us to propose that MC sensing is a generic property of curved membranes rather than a property of the anchoring molecules. We therefore anticipate that MC will promote the redistribution of proteins that are anchored in membranes through other types of hydrophobic moieties.",

author = "Nikos Hatzakis and Bhatia, {Vikram Kj{\o}ller} and Jannik Larsen and Madsen, {Kenneth Lindegaard} and Pierre-Yves Bolinger and Kunding, {Andreas Hjarne} and {Castillo Leon}, {John Jairo} and Ulrik Gether and Per Hedeg{\aa}rd and Dimitrios Stamou",

note = "Keywords: Biotinylation; Fluoresceins; Kinetics; Lipid Bilayers; Liposomes; Membrane Lipids; Membrane Proteins; Membranes; Microscopy, Confocal; Microscopy, Fluorescence; Models, Molecular; Peptides",

year = "2009",

doi = "10.1038/nchembio.213",

language = "English",

volume = "5",

pages = "835--841",

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T1 - How curved membranes recruit amphipathic helices and protein anchoring motifs

AU - Hatzakis, Nikos

AU - Bhatia, Vikram Kjøller

AU - Larsen, Jannik

AU - Madsen, Kenneth Lindegaard

AU - Bolinger, Pierre-Yves

AU - Kunding, Andreas Hjarne

AU - Castillo Leon, John Jairo

AU - Gether, Ulrik

AU - Hedegård, Per

AU - Stamou, Dimitrios

N1 - Keywords: Biotinylation; Fluoresceins; Kinetics; Lipid Bilayers; Liposomes; Membrane Lipids; Membrane Proteins; Membranes; Microscopy, Confocal; Microscopy, Fluorescence; Models, Molecular; Peptides

PY - 2009

Y1 - 2009

N2 - Lipids and several specialized proteins are thought to be able to sense the curvature of membranes (MC). Here we used quantitative fluorescence microscopy to measure curvature-selective binding of amphipathic motifs on single liposomes 50-700 nm in diameter. Our results revealed that sensing is predominantly mediated by a higher density of binding sites on curved membranes instead of higher affinity. We proposed a model based on curvature-induced defects in lipid packing that related these findings to lipid sorting and accurately predicted the existence of a new ubiquitous class of curvature sensors: membrane-anchored proteins. The fact that unrelated structural motifs such as alpha-helices and alkyl chains sense MC led us to propose that MC sensing is a generic property of curved membranes rather than a property of the anchoring molecules. We therefore anticipate that MC will promote the redistribution of proteins that are anchored in membranes through other types of hydrophobic moieties.

AB - Lipids and several specialized proteins are thought to be able to sense the curvature of membranes (MC). Here we used quantitative fluorescence microscopy to measure curvature-selective binding of amphipathic motifs on single liposomes 50-700 nm in diameter. Our results revealed that sensing is predominantly mediated by a higher density of binding sites on curved membranes instead of higher affinity. We proposed a model based on curvature-induced defects in lipid packing that related these findings to lipid sorting and accurately predicted the existence of a new ubiquitous class of curvature sensors: membrane-anchored proteins. The fact that unrelated structural motifs such as alpha-helices and alkyl chains sense MC led us to propose that MC sensing is a generic property of curved membranes rather than a property of the anchoring molecules. We therefore anticipate that MC will promote the redistribution of proteins that are anchored in membranes through other types of hydrophobic moieties.

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JO - Nature Chemical Biology

JF - Nature Chemical Biology

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How curved membranes recruit amphipathic helices and protein anchoring motifs

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