TY - JOUR
T1 - How curved membranes recruit amphipathic helices and protein anchoring motifs
AU - Hatzakis, Nikos
AU - Bhatia, Vikram Kjøller
AU - Larsen, Jannik
AU - Madsen, Kenneth Lindegaard
AU - Bolinger, Pierre-Yves
AU - Kunding, Andreas Hjarne
AU - Castillo Leon, John Jairo
AU - Gether, Ulrik
AU - Hedegård, Per
AU - Stamou, Dimitrios
N1 - Keywords: Biotinylation; Fluoresceins; Kinetics; Lipid Bilayers; Liposomes; Membrane Lipids; Membrane Proteins; Membranes; Microscopy, Confocal; Microscopy, Fluorescence; Models, Molecular; Peptides
PY - 2009
Y1 - 2009
N2 - Lipids and several specialized proteins are thought to be able to sense the curvature of membranes (MC). Here we used quantitative fluorescence microscopy to measure curvature-selective binding of amphipathic motifs on single liposomes 50-700 nm in diameter. Our results revealed that sensing is predominantly mediated by a higher density of binding sites on curved membranes instead of higher affinity. We proposed a model based on curvature-induced defects in lipid packing that related these findings to lipid sorting and accurately predicted the existence of a new ubiquitous class of curvature sensors: membrane-anchored proteins. The fact that unrelated structural motifs such as alpha-helices and alkyl chains sense MC led us to propose that MC sensing is a generic property of curved membranes rather than a property of the anchoring molecules. We therefore anticipate that MC will promote the redistribution of proteins that are anchored in membranes through other types of hydrophobic moieties.
AB - Lipids and several specialized proteins are thought to be able to sense the curvature of membranes (MC). Here we used quantitative fluorescence microscopy to measure curvature-selective binding of amphipathic motifs on single liposomes 50-700 nm in diameter. Our results revealed that sensing is predominantly mediated by a higher density of binding sites on curved membranes instead of higher affinity. We proposed a model based on curvature-induced defects in lipid packing that related these findings to lipid sorting and accurately predicted the existence of a new ubiquitous class of curvature sensors: membrane-anchored proteins. The fact that unrelated structural motifs such as alpha-helices and alkyl chains sense MC led us to propose that MC sensing is a generic property of curved membranes rather than a property of the anchoring molecules. We therefore anticipate that MC will promote the redistribution of proteins that are anchored in membranes through other types of hydrophobic moieties.
U2 - 10.1038/nchembio.213
DO - 10.1038/nchembio.213
M3 - Journal article
C2 - 19749743
SN - 1552-4450
VL - 5
SP - 835
EP - 841
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 11
ER -