HLA-DQA1 typing in Danes by two polymerase chain reaction (PCR) based methods

J B Cowland; H O Madsen; N Morling

HLA-DQA1 typing in Danes by two polymerase chain reaction (PCR) based methods

J B Cowland, H O Madsen, N Morling

Section of Forensic Genetics

9 Citations (Scopus)

Abstract

A total of 280 persons were HLA-DQA1 typed by two different polymerase chain reaction (PCR) based methods; (i) a reverse dot-blot (RDB) method, which can differentiate between six alleles, and (ii) a combined PCR-restriction fragment length polymorphism (PCR-RFLP) and allele-specific amplification (ASA) method, which together recognise eight alleles. In 146 unrelated Danish individuals, the HLA-DQA1 alleles were in Hardy-Weinberg equilibrium. For identity testing, the power of discrimination (PD) of HLA-DQA1 was 0.932 with the RDB method and 0.942 with the PCR-RFLP/ASA method. For paternity testing, the theoretical chance of exclusion in HLA-DQA1 of non-fathers was 0.634 with the RDB method and 0.660 with the PCR-RFLP/ASA method.

Original language	English
Journal	Forensic Science International
Volume	73
Issue number	1
Pages (from-to)	1-13
Number of pages	12
ISSN	0379-0738
Publication status	Published - 1995

Cite this

@article{6b3a6230e4b711deba73000ea68e967b,

title = "HLA-DQA1 typing in Danes by two polymerase chain reaction (PCR) based methods",

abstract = "A total of 280 persons were HLA-DQA1 typed by two different polymerase chain reaction (PCR) based methods; (i) a reverse dot-blot (RDB) method, which can differentiate between six alleles, and (ii) a combined PCR-restriction fragment length polymorphism (PCR-RFLP) and allele-specific amplification (ASA) method, which together recognise eight alleles. In 146 unrelated Danish individuals, the HLA-DQA1 alleles were in Hardy-Weinberg equilibrium. For identity testing, the power of discrimination (PD) of HLA-DQA1 was 0.932 with the RDB method and 0.942 with the PCR-RFLP/ASA method. For paternity testing, the theoretical chance of exclusion in HLA-DQA1 of non-fathers was 0.634 with the RDB method and 0.660 with the PCR-RFLP/ASA method.",

author = "Cowland, {J B} and Madsen, {H O} and N Morling",

note = "Keywords: Alleles; Base Sequence; DNA Primers; Denmark; Gene Amplification; Gene Frequency; HLA-DQ Antigens; Humans; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sequence Alignment",

year = "1995",

language = "English",

volume = "73",

pages = "1--13",

journal = "Forensic Science International",

issn = "0379-0738",

publisher = "Elsevier Ireland Ltd",

number = "1",

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TY - JOUR

T1 - HLA-DQA1 typing in Danes by two polymerase chain reaction (PCR) based methods

AU - Cowland, J B

AU - Madsen, H O

AU - Morling, N

N1 - Keywords: Alleles; Base Sequence; DNA Primers; Denmark; Gene Amplification; Gene Frequency; HLA-DQ Antigens; Humans; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sequence Alignment

PY - 1995

Y1 - 1995

N2 - A total of 280 persons were HLA-DQA1 typed by two different polymerase chain reaction (PCR) based methods; (i) a reverse dot-blot (RDB) method, which can differentiate between six alleles, and (ii) a combined PCR-restriction fragment length polymorphism (PCR-RFLP) and allele-specific amplification (ASA) method, which together recognise eight alleles. In 146 unrelated Danish individuals, the HLA-DQA1 alleles were in Hardy-Weinberg equilibrium. For identity testing, the power of discrimination (PD) of HLA-DQA1 was 0.932 with the RDB method and 0.942 with the PCR-RFLP/ASA method. For paternity testing, the theoretical chance of exclusion in HLA-DQA1 of non-fathers was 0.634 with the RDB method and 0.660 with the PCR-RFLP/ASA method.

AB - A total of 280 persons were HLA-DQA1 typed by two different polymerase chain reaction (PCR) based methods; (i) a reverse dot-blot (RDB) method, which can differentiate between six alleles, and (ii) a combined PCR-restriction fragment length polymorphism (PCR-RFLP) and allele-specific amplification (ASA) method, which together recognise eight alleles. In 146 unrelated Danish individuals, the HLA-DQA1 alleles were in Hardy-Weinberg equilibrium. For identity testing, the power of discrimination (PD) of HLA-DQA1 was 0.932 with the RDB method and 0.942 with the PCR-RFLP/ASA method. For paternity testing, the theoretical chance of exclusion in HLA-DQA1 of non-fathers was 0.634 with the RDB method and 0.660 with the PCR-RFLP/ASA method.

M3 - Journal article

C2 - 7750878

SN - 0379-0738

VL - 73

SP - 1

EP - 13

JO - Forensic Science International

JF - Forensic Science International

IS - 1

ER -

HLA-DQA1 typing in Danes by two polymerase chain reaction (PCR) based methods

Abstract

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