Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils.

Kim Theilgaard-Mönch; Lars C Jacobsen; Thomas Rasmussen; Carsten U Niemann; Lene Udby; Rehannah Borup; Maged Gharib; Peter D Arkwright; Adrian F Gombart; Jero Calafat; Bo T Porse; Niels Borregaard

doi:10.1189/jlb.0105042

Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils.

Kim Theilgaard-Mönch, Lars C Jacobsen, Thomas Rasmussen, Carsten U Niemann, Lene Udby, Rehannah Borup, Maged Gharib, Peter D Arkwright, Adrian F Gombart, Jero Calafat, Bo T Porse, Niels Borregaard

40 Citations (Scopus)

Abstract

Alpha-1-acid glycoprotein (AGP) is an acute-phase protein produced by hepatocytes and secreted into plasma in response to infection/injury. We recently assessed the transcriptional program of terminal granulocytic differentiation by microarray analysis of bone marrow (BM) populations highly enriched in promyelocytes, myelocytes/metamyelocytes (MYs), and BM neutrophils. These analyses demonstrated a transient, high mRNA expression of genuine secondary/tertiary granule proteins and AGP in MYs. In agreement with this, immunocytochemistry revealed the presence of AGP protein and the secondary granule protein lactoferrin in cells from the MY stage and throughout granulocytic differentiation. Immunoelectron microscopy demonstrated the colocalization of AGP and lactoferrin in secondary granules of neutrophils. This finding was substantiated by the failure to detect AGP and lactoferrin in blood cells from a patient with secondary/tertiary (specific) granule deficiency. In addition, Western blot analysis of subcellular fractions isolated from neutrophils revealed that neutrophil-derived AGP, localized in secondary granules, was abundant and highly glycosylated compared with endocytosed, plasma-derived AGP localized in secretory vesicles. Exocytosis studies further demonstrated a marked release of AGP and lactoferrin by activated neutrophils. Finally, induction of CCAAT/enhancer-binding protein (C/EBP)-epsilon in a myeloid cell line was shown to increase AGP transcript levels, indicating that AGP expression in myeloid cells, like in hepatocytes, is partially regulated by members of the C/EBP family. Overall, these findings define AGP as a genuine secondary granule protein of neutrophils. Hence, neutrophils, which constitute the first line of defense, are likely to serve as the primary local source of AGP at sites of infection or injury.

Original language	English
Journal	Journal of Leukocyte Biology
Volume	78
Issue number	2
Pages (from-to)	462-70
Number of pages	8
ISSN	0741-5400
DOIs	https://doi.org/10.1189/jlb.0105042
Publication status	Published - 2005

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10.1189/jlb.0105042

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Theilgaard-Mönch, K., Jacobsen, L. C., Rasmussen, T., Niemann, C. U., Udby, L., Borup, R., Gharib, M., Arkwright, P. D., Gombart, A. F., Calafat, J., Porse, B. T., & Borregaard, N. (2005). Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils. Journal of Leukocyte Biology, 78(2), 462-70. https://doi.org/10.1189/jlb.0105042

Theilgaard-Mönch, K, Jacobsen, LC, Rasmussen, T, Niemann, CU, Udby, L, Borup, R, Gharib, M, Arkwright, PD, Gombart, AF, Calafat, J, Porse, BT & Borregaard, N 2005, 'Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils.', Journal of Leukocyte Biology, vol. 78, no. 2, pp. 462-70. https://doi.org/10.1189/jlb.0105042

@article{dcd6e700588c11dd8d9f000ea68e967b,

title = "Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils.",

abstract = "Alpha-1-acid glycoprotein (AGP) is an acute-phase protein produced by hepatocytes and secreted into plasma in response to infection/injury. We recently assessed the transcriptional program of terminal granulocytic differentiation by microarray analysis of bone marrow (BM) populations highly enriched in promyelocytes, myelocytes/metamyelocytes (MYs), and BM neutrophils. These analyses demonstrated a transient, high mRNA expression of genuine secondary/tertiary granule proteins and AGP in MYs. In agreement with this, immunocytochemistry revealed the presence of AGP protein and the secondary granule protein lactoferrin in cells from the MY stage and throughout granulocytic differentiation. Immunoelectron microscopy demonstrated the colocalization of AGP and lactoferrin in secondary granules of neutrophils. This finding was substantiated by the failure to detect AGP and lactoferrin in blood cells from a patient with secondary/tertiary (specific) granule deficiency. In addition, Western blot analysis of subcellular fractions isolated from neutrophils revealed that neutrophil-derived AGP, localized in secondary granules, was abundant and highly glycosylated compared with endocytosed, plasma-derived AGP localized in secretory vesicles. Exocytosis studies further demonstrated a marked release of AGP and lactoferrin by activated neutrophils. Finally, induction of CCAAT/enhancer-binding protein (C/EBP)-epsilon in a myeloid cell line was shown to increase AGP transcript levels, indicating that AGP expression in myeloid cells, like in hepatocytes, is partially regulated by members of the C/EBP family. Overall, these findings define AGP as a genuine secondary granule protein of neutrophils. Hence, neutrophils, which constitute the first line of defense, are likely to serve as the primary local source of AGP at sites of infection or injury.",

author = "Kim Theilgaard-M{\"o}nch and Jacobsen, {Lars C} and Thomas Rasmussen and Niemann, {Carsten U} and Lene Udby and Rehannah Borup and Maged Gharib and Arkwright, {Peter D} and Gombart, {Adrian F} and Jero Calafat and Porse, {Bo T} and Niels Borregaard",

note = "Keywords: CCAAT-Enhancer-Binding Proteins; Cell Degranulation; Cell Line; Cytoplasmic Granules; Gene Expression Regulation; Granulocyte Precursor Cells; Hepatocytes; Humans; Immunochemistry; Lactoferrin; Liver; Microscopy, Electron, Transmission; Neutrophil Activation; Neutrophils; Orosomucoid; RNA, Messenger",

year = "2005",

doi = "10.1189/jlb.0105042",

language = "English",

volume = "78",

pages = "462--70",

journal = "Journal of Leukocyte Biology",

issn = "0741-5400",

publisher = "Federation of American Societies for Experimental Biology",

number = "2",

}

TY - JOUR

T1 - Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils.

AU - Theilgaard-Mönch, Kim

AU - Jacobsen, Lars C

AU - Rasmussen, Thomas

AU - Niemann, Carsten U

AU - Udby, Lene

AU - Borup, Rehannah

AU - Gharib, Maged

AU - Arkwright, Peter D

AU - Gombart, Adrian F

AU - Calafat, Jero

AU - Porse, Bo T

AU - Borregaard, Niels

N1 - Keywords: CCAAT-Enhancer-Binding Proteins; Cell Degranulation; Cell Line; Cytoplasmic Granules; Gene Expression Regulation; Granulocyte Precursor Cells; Hepatocytes; Humans; Immunochemistry; Lactoferrin; Liver; Microscopy, Electron, Transmission; Neutrophil Activation; Neutrophils; Orosomucoid; RNA, Messenger

PY - 2005

Y1 - 2005

N2 - Alpha-1-acid glycoprotein (AGP) is an acute-phase protein produced by hepatocytes and secreted into plasma in response to infection/injury. We recently assessed the transcriptional program of terminal granulocytic differentiation by microarray analysis of bone marrow (BM) populations highly enriched in promyelocytes, myelocytes/metamyelocytes (MYs), and BM neutrophils. These analyses demonstrated a transient, high mRNA expression of genuine secondary/tertiary granule proteins and AGP in MYs. In agreement with this, immunocytochemistry revealed the presence of AGP protein and the secondary granule protein lactoferrin in cells from the MY stage and throughout granulocytic differentiation. Immunoelectron microscopy demonstrated the colocalization of AGP and lactoferrin in secondary granules of neutrophils. This finding was substantiated by the failure to detect AGP and lactoferrin in blood cells from a patient with secondary/tertiary (specific) granule deficiency. In addition, Western blot analysis of subcellular fractions isolated from neutrophils revealed that neutrophil-derived AGP, localized in secondary granules, was abundant and highly glycosylated compared with endocytosed, plasma-derived AGP localized in secretory vesicles. Exocytosis studies further demonstrated a marked release of AGP and lactoferrin by activated neutrophils. Finally, induction of CCAAT/enhancer-binding protein (C/EBP)-epsilon in a myeloid cell line was shown to increase AGP transcript levels, indicating that AGP expression in myeloid cells, like in hepatocytes, is partially regulated by members of the C/EBP family. Overall, these findings define AGP as a genuine secondary granule protein of neutrophils. Hence, neutrophils, which constitute the first line of defense, are likely to serve as the primary local source of AGP at sites of infection or injury.

AB - Alpha-1-acid glycoprotein (AGP) is an acute-phase protein produced by hepatocytes and secreted into plasma in response to infection/injury. We recently assessed the transcriptional program of terminal granulocytic differentiation by microarray analysis of bone marrow (BM) populations highly enriched in promyelocytes, myelocytes/metamyelocytes (MYs), and BM neutrophils. These analyses demonstrated a transient, high mRNA expression of genuine secondary/tertiary granule proteins and AGP in MYs. In agreement with this, immunocytochemistry revealed the presence of AGP protein and the secondary granule protein lactoferrin in cells from the MY stage and throughout granulocytic differentiation. Immunoelectron microscopy demonstrated the colocalization of AGP and lactoferrin in secondary granules of neutrophils. This finding was substantiated by the failure to detect AGP and lactoferrin in blood cells from a patient with secondary/tertiary (specific) granule deficiency. In addition, Western blot analysis of subcellular fractions isolated from neutrophils revealed that neutrophil-derived AGP, localized in secondary granules, was abundant and highly glycosylated compared with endocytosed, plasma-derived AGP localized in secretory vesicles. Exocytosis studies further demonstrated a marked release of AGP and lactoferrin by activated neutrophils. Finally, induction of CCAAT/enhancer-binding protein (C/EBP)-epsilon in a myeloid cell line was shown to increase AGP transcript levels, indicating that AGP expression in myeloid cells, like in hepatocytes, is partially regulated by members of the C/EBP family. Overall, these findings define AGP as a genuine secondary granule protein of neutrophils. Hence, neutrophils, which constitute the first line of defense, are likely to serve as the primary local source of AGP at sites of infection or injury.

U2 - 10.1189/jlb.0105042

DO - 10.1189/jlb.0105042

M3 - Journal article

C2 - 15941779

SN - 0741-5400

VL - 78

SP - 462

EP - 470

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

IS - 2

ER -

Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils.

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