High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae)

Sileshi Gizachew Wubshet; Henrique H. Moresco; Yousof Tahtah; Inês M. C. Brighente; Dan Stærk

doi:10.1016/j.phytochem.2015.04.004

High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae)

Sileshi Gizachew Wubshet, Henrique H. Moresco, Yousof Tahtah, Inês M. C. Brighente, Dan Stærk

28 Citations (Scopus)

Abstract

Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20 years. T2D is associated with severe and lifethreatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution a-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-D-(6"-galloyl)galactopyranoside, kaempferol 3-O-β-D-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(2",3"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(3",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, and 4-O-(2",3",4"-O-triacetyl-α-L-rhamnopyranosyl)ellagic acid were identified.

Original language	English
Journal	Phytochemistry
Volume	116
Pages (from-to)	246-252
Number of pages	7
ISSN	0031-9422
DOIs	https://doi.org/10.1016/j.phytochem.2015.04.004
Publication status	Published - 2015

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10.1016/j.phytochem.2015.04.004

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High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae). / Wubshet, Sileshi Gizachew; Moresco, Henrique H.; Tahtah, Yousof et al.
In: Phytochemistry, Vol. 116, 2015, p. 246-252.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae)",

abstract = "Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20 years. T2D is associated with severe and lifethreatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution a-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-D-(6{"}-galloyl)galactopyranoside, kaempferol 3-O-β-D-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2{"},4{"}-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(2{"},3{"}-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(3{"},4{"}-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, and 4-O-(2{"},3{"},4{"}-O-triacetyl-α-L-rhamnopyranosyl)ellagic acid were identified.",

author = "Wubshet, {Sileshi Gizachew} and Moresco, {Henrique H.} and Yousof Tahtah and Brighente, {In{\^e}s M. C.} and Dan St{\ae}rk",

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AU - Wubshet, Sileshi Gizachew

AU - Moresco, Henrique H.

AU - Tahtah, Yousof

AU - Brighente, Inês M. C.

AU - Stærk, Dan

PY - 2015

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N2 - Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20 years. T2D is associated with severe and lifethreatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution a-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-D-(6"-galloyl)galactopyranoside, kaempferol 3-O-β-D-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(2",3"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(3",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, and 4-O-(2",3",4"-O-triacetyl-α-L-rhamnopyranosyl)ellagic acid were identified.

AB - Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20 years. T2D is associated with severe and lifethreatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution a-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-D-(6"-galloyl)galactopyranoside, kaempferol 3-O-β-D-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(2",3"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(3",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, and 4-O-(2",3",4"-O-triacetyl-α-L-rhamnopyranosyl)ellagic acid were identified.

U2 - 10.1016/j.phytochem.2015.04.004

DO - 10.1016/j.phytochem.2015.04.004

M3 - Journal article

C2 - 25935545

SN - 0031-9422

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SP - 246

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JO - Phytochemistry

JF - Phytochemistry

ER -

High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae)

Abstract

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