High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae)

TY - JOUR

T1 - High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae)

AU - Wubshet, Sileshi Gizachew

AU - Moresco, Henrique H.

AU - Tahtah, Yousof

AU - Brighente, Inês M. C.

AU - Stærk, Dan

PY - 2015

Y1 - 2015

N2 - Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20 years. T2D is associated with severe and lifethreatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution a-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-D-(6"-galloyl)galactopyranoside, kaempferol 3-O-β-D-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(2",3"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(3",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, and 4-O-(2",3",4"-O-triacetyl-α-L-rhamnopyranosyl)ellagic acid were identified.

AB - Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20 years. T2D is associated with severe and lifethreatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution a-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-D-(6"-galloyl)galactopyranoside, kaempferol 3-O-β-D-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(2",3"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, 4-O-(3",4"-O-diacetyl-α-L-rhamnopyranosyl)ellagic acid, and 4-O-(2",3",4"-O-triacetyl-α-L-rhamnopyranosyl)ellagic acid were identified.

U2 - 10.1016/j.phytochem.2015.04.004

DO - 10.1016/j.phytochem.2015.04.004

M3 - Journal article

C2 - 25935545

SN - 0031-9422

VL - 116

SP - 246

EP - 252

JO - Phytochemistry

JF - Phytochemistry

ER -