High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

Ida Elisabeth Johansen; Ying Liu; Bodil Jorgensen; Eric Paul Bennett; Erik Andreasson; Kare L. Nielsen; Andreas Blennow; Bent Larsen Petersen

doi:10.1038/s41598-019-54126-w

High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

Ida Elisabeth Johansen, Ying Liu, Bodil Jorgensen, Eric Paul Bennett, Erik Andreasson, Kare L. Nielsen, Andreas Blennow, Bent Larsen Petersen

10 Citations (Scopus)

Abstract

CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

Original language	English
Journal	Scientific Reports
Volume	9
ISSN	2045-2322
DOIs	https://doi.org/10.1038/s41598-019-54126-w
Publication status	Published - 1 Dec 2019

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title = "High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato",

abstract = "CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.",

author = "Johansen, {Ida Elisabeth} and Ying Liu and Bodil Jorgensen and Bennett, {Eric Paul} and Erik Andreasson and Nielsen, {Kare L.} and Andreas Blennow and Petersen, {Bent Larsen}",

year = "2019",

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doi = "10.1038/s41598-019-54126-w",

language = "English",

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T1 - High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

AU - Johansen, Ida Elisabeth

AU - Liu, Ying

AU - Jorgensen, Bodil

AU - Bennett, Eric Paul

AU - Andreasson, Erik

AU - Nielsen, Kare L.

AU - Blennow, Andreas

AU - Petersen, Bent Larsen

PY - 2019/12/1

Y1 - 2019/12/1

N2 - CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

AB - CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

U2 - 10.1038/s41598-019-54126-w

DO - 10.1038/s41598-019-54126-w

M3 - Journal article

C2 - 31776399

SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

ER -

High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

Abstract

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