Heat treatment and irradiation reduce anti-bacterial and immune-modulatory properties of bovine colostrum

Duc Ninh Nguyen; Andrew J. Currie; Shuqiang Ren; Stine B. Bering; Per T. Sangild

doi:10.1016/j.jff.2019.04.012

Heat treatment and irradiation reduce anti-bacterial and immune-modulatory properties of bovine colostrum

Duc Ninh Nguyen, Andrew J. Currie, Shuqiang Ren, Stine B. Bering, Per T. Sangild^*

^*Corresponding author for this work

4 Citations (Scopus)

Abstract

Colostrum contains bioactive components protecting the newborn intestine against bacteria. It is unclear how to optimize processing conditions with highest product bioactivity. Non-pasteurized (BC00), standard-pasteurized (72 °C-15 s, BC72), gently-pasteurized bovine colostrums without (63 °C-30 min, BC63) and with gamma-irradiation (BC63g) were tested for effects on bacterial growth inhibition (Escherichia coli, Staphylococcus aureus and S. epidermidis), intestinal epithelial cell (IEC) proliferation and cytokine secretion in vitro. Thermal processing decreased endogenous bacteria and IgG levels. All BCs inhibited bacterial growth 1–2 h after inoculation, but only BC00, BC63 and BC63g retained activity after 4–24 h. After 4 h, the activity against S. epidermidis of BC63g was lower than BC00 but still potent when mixed with formula. All BCs stimulated IEC proliferation, with the most pronounced responses for BC00. Only BC00 and BC63 increased IL-8 secretion in lipopolysaccharide-stimulated IECs. Thermal processing reduced bioactivity and combined gentle pasteurization and gamma-irradiation improved BC sterility and bioactivity, relative to standard pasteurization.

Original language	English
Journal	Journal of Functional Foods
Volume	57
Pages (from-to)	182-189
Number of pages	8
ISSN	1756-4646
DOIs	https://doi.org/10.1016/j.jff.2019.04.012
Publication status	Published - 2019

Keywords

Antimicrobial activity
Bacterial growth inhibition
Bovine colostrum
Pasteurization
Preterm infants

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10.1016/j.jff.2019.04.012

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@article{d621ba11c3f64f83b884162b7649e4fc,

title = "Heat treatment and irradiation reduce anti-bacterial and immune-modulatory properties of bovine colostrum",

abstract = "Colostrum contains bioactive components protecting the newborn intestine against bacteria. It is unclear how to optimize processing conditions with highest product bioactivity. Non-pasteurized (BC00), standard-pasteurized (72 °C-15 s, BC72), gently-pasteurized bovine colostrums without (63 °C-30 min, BC63) and with gamma-irradiation (BC63g) were tested for effects on bacterial growth inhibition (Escherichia coli, Staphylococcus aureus and S. epidermidis), intestinal epithelial cell (IEC) proliferation and cytokine secretion in vitro. Thermal processing decreased endogenous bacteria and IgG levels. All BCs inhibited bacterial growth 1–2 h after inoculation, but only BC00, BC63 and BC63g retained activity after 4–24 h. After 4 h, the activity against S. epidermidis of BC63g was lower than BC00 but still potent when mixed with formula. All BCs stimulated IEC proliferation, with the most pronounced responses for BC00. Only BC00 and BC63 increased IL-8 secretion in lipopolysaccharide-stimulated IECs. Thermal processing reduced bioactivity and combined gentle pasteurization and gamma-irradiation improved BC sterility and bioactivity, relative to standard pasteurization.",

keywords = "Antimicrobial activity, Bacterial growth inhibition, Bovine colostrum, Pasteurization, Preterm infants",

author = "Nguyen, {Duc Ninh} and Currie, {Andrew J.} and Shuqiang Ren and Bering, {Stine B.} and Sangild, {Per T.}",

year = "2019",

doi = "10.1016/j.jff.2019.04.012",

language = "English",

volume = "57",

pages = "182--189",

journal = "Journal of Functional Foods",

issn = "1756-4646",

publisher = "Elsevier",

}

TY - JOUR

T1 - Heat treatment and irradiation reduce anti-bacterial and immune-modulatory properties of bovine colostrum

AU - Nguyen, Duc Ninh

AU - Currie, Andrew J.

AU - Ren, Shuqiang

AU - Bering, Stine B.

AU - Sangild, Per T.

PY - 2019

Y1 - 2019

N2 - Colostrum contains bioactive components protecting the newborn intestine against bacteria. It is unclear how to optimize processing conditions with highest product bioactivity. Non-pasteurized (BC00), standard-pasteurized (72 °C-15 s, BC72), gently-pasteurized bovine colostrums without (63 °C-30 min, BC63) and with gamma-irradiation (BC63g) were tested for effects on bacterial growth inhibition (Escherichia coli, Staphylococcus aureus and S. epidermidis), intestinal epithelial cell (IEC) proliferation and cytokine secretion in vitro. Thermal processing decreased endogenous bacteria and IgG levels. All BCs inhibited bacterial growth 1–2 h after inoculation, but only BC00, BC63 and BC63g retained activity after 4–24 h. After 4 h, the activity against S. epidermidis of BC63g was lower than BC00 but still potent when mixed with formula. All BCs stimulated IEC proliferation, with the most pronounced responses for BC00. Only BC00 and BC63 increased IL-8 secretion in lipopolysaccharide-stimulated IECs. Thermal processing reduced bioactivity and combined gentle pasteurization and gamma-irradiation improved BC sterility and bioactivity, relative to standard pasteurization.

AB - Colostrum contains bioactive components protecting the newborn intestine against bacteria. It is unclear how to optimize processing conditions with highest product bioactivity. Non-pasteurized (BC00), standard-pasteurized (72 °C-15 s, BC72), gently-pasteurized bovine colostrums without (63 °C-30 min, BC63) and with gamma-irradiation (BC63g) were tested for effects on bacterial growth inhibition (Escherichia coli, Staphylococcus aureus and S. epidermidis), intestinal epithelial cell (IEC) proliferation and cytokine secretion in vitro. Thermal processing decreased endogenous bacteria and IgG levels. All BCs inhibited bacterial growth 1–2 h after inoculation, but only BC00, BC63 and BC63g retained activity after 4–24 h. After 4 h, the activity against S. epidermidis of BC63g was lower than BC00 but still potent when mixed with formula. All BCs stimulated IEC proliferation, with the most pronounced responses for BC00. Only BC00 and BC63 increased IL-8 secretion in lipopolysaccharide-stimulated IECs. Thermal processing reduced bioactivity and combined gentle pasteurization and gamma-irradiation improved BC sterility and bioactivity, relative to standard pasteurization.

KW - Antimicrobial activity

KW - Bacterial growth inhibition

KW - Bovine colostrum

KW - Pasteurization

KW - Preterm infants

U2 - 10.1016/j.jff.2019.04.012

DO - 10.1016/j.jff.2019.04.012

M3 - Journal article

AN - SCOPUS:85064116728

SN - 1756-4646

VL - 57

SP - 182

EP - 189

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

Heat treatment and irradiation reduce anti-bacterial and immune-modulatory properties of bovine colostrum

Abstract

Keywords

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