Grafted biomembranes containing membrane proteins – the case of the leucine transporter

Vivien Jagalski; Robert D. Barker; Mikkel Boas Thygesen; Kamil Gotfryd; Mie Barthold Krüger; Lei Shi; Selma Maric; Nicolas Emile Bovet; Martine Moulin; Michael Haertlein; Thomas Günther-Pomorski; Claus Juul Løland; Marite Cardenas Gomez

doi:10.1039/c5sm01490e

Grafted biomembranes containing membrane proteins – the case of the leucine transporter

Vivien Jagalski, Robert D. Barker, Mikkel Boas Thygesen, Kamil Gotfryd, Mie Barthold Krüger, Lei Shi, Selma Maric, Nicolas Emile Bovet, Martine Moulin, Michael Haertlein, Thomas Günther-Pomorski, Claus Juul Løland, Marite Cardenas Gomez

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Abstract

Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.

Original language	English
Journal	Soft Matter
Volume	11
Issue number	39
Pages (from-to)	7707-7711
Number of pages	5
ISSN	1744-683X
DOIs	https://doi.org/10.1039/c5sm01490e
Publication status	Published - 19 Aug 2015

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Jagalski_2015_Grafted_biomembranes_containingFinal published version, 1.95 MBLicence: CC BY

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title = "Grafted biomembranes containing membrane proteins – the case of the leucine transporter",

abstract = "Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.",

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T1 - Grafted biomembranes containing membrane proteins – the case of the leucine transporter

AU - Jagalski, Vivien

AU - Barker, Robert D.

AU - Thygesen, Mikkel Boas

AU - Gotfryd, Kamil

AU - Krüger, Mie Barthold

AU - Shi, Lei

AU - Maric, Selma

AU - Bovet, Nicolas Emile

AU - Moulin, Martine

AU - Haertlein, Michael

AU - Günther-Pomorski, Thomas

AU - Løland, Claus Juul

AU - Cardenas Gomez, Marite

PY - 2015/8/19

Y1 - 2015/8/19

N2 - Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.

AB - Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.

U2 - 10.1039/c5sm01490e

DO - 10.1039/c5sm01490e

M3 - Journal article

C2 - 26325086

SN - 1744-683X

VL - 11

SP - 7707

EP - 7711

JO - Soft Matter

JF - Soft Matter

IS - 39

ER -

Grafted biomembranes containing membrane proteins – the case of the leucine transporter

Abstract

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