Abstract
We developed specific antibodies and RIAs for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2), two predicted products of the glucagon gene, and studied the occurrence, nature, and secretion of immunoreactive GLP-1 and GLP-2 in pig pancreas and small intestine. Immunoreactive GLP-1 and GLP-2 were identified in glucagon-producing cells of the pancreatic islets, and in glicentin-producing cells of the small intestine. Immunoreactive GLP-1 and 2 in intestinal extracts corresponded in molecular size to peptides synthesized according to the predicted structure. By reverse phase HPLC, intestinal and synthetic GLP-1 behaved similarly, whereas synthetic and intestinal GLP-2 differed. Pancreatic extracts contained a large peptide with both GLP-1 and GLP-2 immunoreactivity. Secretion was studied using isolated perfused pig pancreas during arginine stimulation, and isolated perfused pig ileum during either luminal glucose stimulation or vascular administration of the neuropeptide, gastrin-releasing peptide (GRP). Immunoreactive GLP-1 and GLP-2 were secreted in parallel with pancreatic glucagon and intestinal glicentin. The molecular forms of secreted immunoreactive GLP-1 and 2 corresponded to those identified in the tissue extracts.
| Original language | English |
|---|---|
| Journal | Endocrinology |
| Volume | 119 |
| Issue number | 4 |
| Pages (from-to) | 1467-75 |
| Number of pages | 9 |
| ISSN | 0013-7227 |
| Publication status | Published - Oct 1986 |
Keywords
- Animals
- Arginine
- Gastrin-Releasing Peptide
- Glucagon
- Glucagon-Like Peptide 1
- Glucagon-Like Peptide 2
- Glucose
- Histocytochemistry
- Immunoenzyme Techniques
- Intestine, Small
- Pancreas
- Peptides
- Proglucagon
- Protein Precursors
- Swine