gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

Jens Bo Andersen; Arne Heydorn; Morten Hentzer; Leo Eberl; O Geisenberger; B B Christensen; Søren Molin; M Givskov

doi:10.1128/AEM.67.2.575-585.2001

gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

Jens Bo Andersen, Arne Heydorn, Morten Hentzer, Leo Eberl, O Geisenberger, B B Christensen, Søren Molin, M Givskov

260 Citations (Scopus)

Abstract

In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	67
Issue number	2
Pages (from-to)	575-85
Number of pages	11
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.67.2.575-585.2001
Publication status	Published - 2001
Externally published	Yes

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10.1128/AEM.67.2.575-585.2001

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title = "gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication",

abstract = "In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.",

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year = "2001",

doi = "10.1128/AEM.67.2.575-585.2001",

language = "English",

volume = "67",

pages = "575--85",

journal = "Applied and Environmental Microbiology",

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publisher = "American Society for Microbiology",

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T1 - gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

AU - Andersen, Jens Bo

AU - Heydorn, Arne

AU - Hentzer, Morten

AU - Eberl, Leo

AU - Geisenberger, O

AU - Christensen, B B

AU - Molin, Søren

AU - Givskov, M

PY - 2001

Y1 - 2001

N2 - In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

AB - In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

U2 - 10.1128/AEM.67.2.575-585.2001

DO - 10.1128/AEM.67.2.575-585.2001

M3 - Journal article

C2 - 11157219

SN - 0099-2240

VL - 67

SP - 575

EP - 585

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 2

ER -

gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

Abstract

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