gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

TY - JOUR

T1 - gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

AU - Andersen, Jens Bo

AU - Heydorn, Arne

AU - Hentzer, Morten

AU - Eberl, Leo

AU - Geisenberger, O

AU - Christensen, B B

AU - Molin, Søren

AU - Givskov, M

PY - 2001

Y1 - 2001

N2 - In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

AB - In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

U2 - 10.1128/AEM.67.2.575-585.2001

DO - 10.1128/AEM.67.2.575-585.2001

M3 - Journal article

C2 - 11157219

SN - 0099-2240

VL - 67

SP - 575

EP - 585

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 2

ER -