Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle

Lea Hüche Larsen; Sofie Sandø-Pedersen; Laura Kofoed Hvidsten Ørstrup; Niels Grunnet; Bjørn Quistorff; Ole Hartvig Mortensen

doi:10.1007/978-94-024-1079-2_34

Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle

Lea Hüche Larsen, Sofie Sandø-Pedersen, Laura Kofoed Hvidsten Ørstrup, Niels Grunnet, Bjørn Quistorff, Ole Hartvig Mortensen

2 Citations (Scopus)

Abstract

Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.

Original language	English
Title of host publication	Taurine 10
Editors	Dong-Hee Lee, Stephen W. Schaffer, Eunkyue Park, Ha Won Kim
Number of pages	21
Volume	975
Publisher	Springer
Publication date	2017
Pages	413-433
ISBN (Print)	978-94-024-1077-8
ISBN (Electronic)	978-94-024-1079-2
DOIs	https://doi.org/10.1007/978-94-024-1079-2_34
Publication status	Published - 2017

Series	Advances in Experimental Medicine and Biology
Number	975
ISSN	0065-2598

Access to Document

10.1007/978-94-024-1079-2_34

Cite this

Larsen, L. H., Sandø-Pedersen, S., Ørstrup, L. K. H., Grunnet, N., Quistorff, B., & Mortensen, O. H. (2017). Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. In D-H. Lee, S. W. Schaffer, E. Park, & H. W. Kim (Eds.), Taurine 10 (Vol. 975, pp. 413-433). Springer. Advances in Experimental Medicine and Biology No. 975 https://doi.org/10.1007/978-94-024-1079-2_34

Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. / Larsen, Lea Hüche; Sandø-Pedersen, Sofie; Ørstrup, Laura Kofoed Hvidsten et al.

Taurine 10. ed. / Dong-Hee Lee; Stephen W. Schaffer; Eunkyue Park; Ha Won Kim. Vol. 975 Springer, 2017. p. 413-433 (Advances in Experimental Medicine and Biology; No. 975).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Larsen, LH, Sandø-Pedersen, S, Ørstrup, LKH, Grunnet, N, Quistorff, B & Mortensen, OH 2017, Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. in D-H Lee, SW Schaffer, E Park & HW Kim (eds), Taurine 10. vol. 975, Springer, Advances in Experimental Medicine and Biology, no. 975, pp. 413-433. https://doi.org/10.1007/978-94-024-1079-2_34

Larsen LH, Sandø-Pedersen S, Ørstrup LKH, Grunnet N, Quistorff B , Mortensen OH. Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. In Lee D-H, Schaffer SW, Park E, Kim HW, editors, Taurine 10. Vol. 975. Springer. 2017. p. 413-433. (Advances in Experimental Medicine and Biology; No. 975). doi: 10.1007/978-94-024-1079-2_34

Larsen, Lea Hüche ; Sandø-Pedersen, Sofie ; Ørstrup, Laura Kofoed Hvidsten et al. / Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. Taurine 10. editor / Dong-Hee Lee ; Stephen W. Schaffer ; Eunkyue Park ; Ha Won Kim. Vol. 975 Springer, 2017. pp. 413-433 (Advances in Experimental Medicine and Biology; No. 975).

@inbook{4cbad1a68dfa403d92d552a0de8c2ecd,

title = "Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle",

abstract = "Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.",

author = "Larsen, {Lea H{\"u}che} and Sofie Sand{\o}-Pedersen and {\O}rstrup, {Laura Kofoed Hvidsten} and Niels Grunnet and Bj{\o}rn Quistorff and Mortensen, {Ole Hartvig}",

year = "2017",

doi = "10.1007/978-94-024-1079-2_34",

language = "English",

isbn = "978-94-024-1077-8",

volume = "975",

series = "Advances in Experimental Medicine and Biology",

publisher = "Springer",

number = "975",

pages = "413--433",

editor = "Lee, {Dong-Hee } and Schaffer, {Stephen W. } and Park, {Eunkyue } and Kim, {Ha Won }",

booktitle = "Taurine 10",

}

TY - CHAP

T1 - Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle

AU - Larsen, Lea Hüche

AU - Sandø-Pedersen, Sofie

AU - Ørstrup, Laura Kofoed Hvidsten

AU - Grunnet, Niels

AU - Quistorff, Bjørn

AU - Mortensen, Ole Hartvig

PY - 2017

Y1 - 2017

N2 - Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.

AB - Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.

U2 - 10.1007/978-94-024-1079-2_34

DO - 10.1007/978-94-024-1079-2_34

M3 - Book chapter

C2 - 28849472

SN - 978-94-024-1077-8

VL - 975

T3 - Advances in Experimental Medicine and Biology

SP - 413

EP - 433

BT - Taurine 10

A2 - Lee, Dong-Hee

A2 - Schaffer, Stephen W.

A2 - Park, Eunkyue

A2 - Kim, Ha Won

PB - Springer

ER -

Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle

Abstract

Access to Document

Fingerprint

Cite this