Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle

Lea Hüche Larsen; Sofie Sandø-Pedersen; Laura Kofoed Hvidsten Ørstrup; Niels Grunnet; Bjørn Quistorff; Ole Hartvig Mortensen

doi:10.1007/978-94-024-1079-2_34

Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle

Lea Hüche Larsen, Sofie Sandø-Pedersen, Laura Kofoed Hvidsten Ørstrup, Niels Grunnet, Bjørn Quistorff, Ole Hartvig Mortensen

2 Citationer (Scopus)

Abstract

Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.

Originalsprog	Engelsk
Titel	Taurine 10
Redaktører	Dong-Hee Lee, Stephen W. Schaffer, Eunkyue Park, Ha Won Kim
Antal sider	21
Vol/bind	975
Forlag	Springer
Publikationsdato	2017
Sider	413-433
ISBN (Trykt)	978-94-024-1077-8
ISBN (Elektronisk)	978-94-024-1079-2
DOI	https://doi.org/10.1007/978-94-024-1079-2_34
Status	Udgivet - 2017

Navn	Advances in Experimental Medicine and Biology
Nummer	975
ISSN	0065-2598

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10.1007/978-94-024-1079-2_34

Citationsformater

Larsen, L. H., Sandø-Pedersen, S., Ørstrup, L. K. H., Grunnet, N., Quistorff, B., & Mortensen, O. H. (2017). Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. I D-H. Lee, S. W. Schaffer, E. Park, & H. W. Kim (red.), Taurine 10 (Bind 975, s. 413-433). Springer. Advances in Experimental Medicine and Biology Nr. 975 https://doi.org/10.1007/978-94-024-1079-2_34

Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. / Larsen, Lea Hüche; Sandø-Pedersen, Sofie; Ørstrup, Laura Kofoed Hvidsten et al.

Taurine 10. red. / Dong-Hee Lee; Stephen W. Schaffer; Eunkyue Park; Ha Won Kim. Bind 975 Springer, 2017. s. 413-433 (Advances in Experimental Medicine and Biology; Nr. 975).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › peer review

Larsen, LH, Sandø-Pedersen, S, Ørstrup, LKH, Grunnet, N, Quistorff, B & Mortensen, OH 2017, Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. i D-H Lee, SW Schaffer, E Park & HW Kim (red), Taurine 10. bind 975, Springer, Advances in Experimental Medicine and Biology, nr. 975, s. 413-433. https://doi.org/10.1007/978-94-024-1079-2_34

Larsen LH, Sandø-Pedersen S, Ørstrup LKH, Grunnet N, Quistorff B , Mortensen OH. Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. I Lee D-H, Schaffer SW, Park E, Kim HW, red., Taurine 10. Bind 975. Springer. 2017. s. 413-433. (Advances in Experimental Medicine and Biology; Nr. 975). doi: 10.1007/978-94-024-1079-2_34

Larsen, Lea Hüche ; Sandø-Pedersen, Sofie ; Ørstrup, Laura Kofoed Hvidsten et al. / Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle. Taurine 10. red. / Dong-Hee Lee ; Stephen W. Schaffer ; Eunkyue Park ; Ha Won Kim. Bind 975 Springer, 2017. s. 413-433 (Advances in Experimental Medicine and Biology; Nr. 975).

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abstract = "Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.",

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AU - Grunnet, Niels

AU - Quistorff, Bjørn

AU - Mortensen, Ole Hartvig

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N2 - Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.

AB - Taurine ameliorates changes occurring in newborn skeletal muscle as a result of gestational protein restriction in C57BL/6 mice, but taurine supplementation effects may be exaggerated in C57BL/6 mice due to their inherent excessive taurinuria.We examined if maternal taurine supplementation could ameliorate changes in gene expression levels, properties of mitochondria, myogenesis, and nutrient transport and sensing, in male newborn skeletal muscle caused by a maternal low protein (LP) diet in Wistar rats.LP diet resulted in an 11% non-significant decrease in birth weight, which was not rescued by taurine supplementation (LP-Tau). LP-Tau offspring had significantly lower birth weight compared to controls. Gene expression profiling revealed 895 significantly changed genes, mainly an LP-induced down-regulation of genes involved in protein translation. Taurine fully or partially rescued 32% of these changes, but with no distinct pattern as to which genes were rescued.Skeletal muscle taurine content in LP-Tau offspring was increased, but no changes in mRNA levels of the taurine synthesis pathway were observed. Taurine transporter mRNA levels, but not protein levels, were increased by LP diet.Nutrient sensing signaling pathways were largely unaffected in LP or LP-Tau groups, although taurine supplementation caused a decrease in total Akt and AMPK protein levels. PAT4 amino acid transporter mRNA was increased by LP, and normalized by taurine supplementation.In conclusion, gestational protein restriction in rats decreased genes involved in protein translation in newborn skeletal muscle and led to changes in nutrient transporters. Taurine partly rescued these changes, hence underscoring the importance of taurine in development.

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ER -

Gestational Protein Restriction in Wistar Rats; Effect of Taurine Supplementation on Properties of Newborn Skeletal Muscle

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