Genomic and immunohistochemical characterisation of a lacrimal gland oncocytoma and review of literature

Lauge Hjorth Mikkelsen; Simon Andreasen; Linea Cicilie Melchior; Marta Persson; Jeppe Dyrberg Andersen; Vania Pereira; Peter Bjerre Toft; Niels Morling; Göran Stenman; Steffen Heegaard

doi:10.3892/ol.2017.6713

Genomic and immunohistochemical characterisation of a lacrimal gland oncocytoma and review of literature

Lauge Hjorth Mikkelsen, Simon Andreasen, Linea Cicilie Melchior, Marta Persson, Jeppe Dyrberg Andersen, Vania Pereira, Peter Bjerre Toft, Niels Morling, Göran Stenman, Steffen Heegaard

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Abstract

The aim of the present study was to report the genetic and immunohistochemical profile of a rare case of lacrimal gland oncocytoma. A 20-year-old male underwent magnetic resonance imaging (MRI) due to viral encephalitis. Notably, the MRI revealed a multicystic tumor in the left lacrimal gland. A lateral orbitotomy was performed and the tumor was completely excised. Four months following surgery, the patient was free of symptoms. Histopathologically, the tumor was composed of large, eosinophilic and polyhedral cells with small round nuclei. The tumor cells stained strongly for antimitochondrial antibody MU213-UC, cytokeratin (CK) 5/6, CK 7, CK 17, CK 8/18 and CK 19. The final diagnosis was an oncocytoma of the lacrimal gland without any signs of malignancy. Array-based comparative genomic hybridisation demonstrated a gain of one copy of chromosome 8 and loss of one copy of chromosome 22 as the sole genomic imbalances. These chromosomal alterations have not previously been identified in oncocytoma and may be specific to lacrimal gland oncocytoma. Sequencing of the mitochondrial genome demonstrated multiple alterations of the NADH-ubiquinone oxidoreductase chain 5 (ND5) gene involved in mitochondrial oxidative phosphorylation. This may support the notion of a common genetic background of oncocytic lesions in the lacrimal gland and other anatomical sites.

Original language	English
Journal	Oncology Letters
Volume	14
Issue number	4
Pages (from-to)	4176-4182
ISSN	1792-1074
DOIs	https://doi.org/10.3892/ol.2017.6713
Publication status	Published - Oct 2017

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Genomic and immunohistochemical characterisation of a lacrimal gland oncocytoma and review of literatureFinal published version, 1.15 MBLicence: CC BY-NC-ND

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@article{94a1e491002e40f1a5c04fb310498198,

title = "Genomic and immunohistochemical characterisation of a lacrimal gland oncocytoma and review of literature",

abstract = "The aim of the present study was to report the genetic and immunohistochemical profile of a rare case of lacrimal gland oncocytoma. A 20-year-old male underwent magnetic resonance imaging (MRI) due to viral encephalitis. Notably, the MRI revealed a multicystic tumor in the left lacrimal gland. A lateral orbitotomy was performed and the tumor was completely excised. Four months following surgery, the patient was free of symptoms. Histopathologically, the tumor was composed of large, eosinophilic and polyhedral cells with small round nuclei. The tumor cells stained strongly for antimitochondrial antibody MU213-UC, cytokeratin (CK) 5/6, CK 7, CK 17, CK 8/18 and CK 19. The final diagnosis was an oncocytoma of the lacrimal gland without any signs of malignancy. Array-based comparative genomic hybridisation demonstrated a gain of one copy of chromosome 8 and loss of one copy of chromosome 22 as the sole genomic imbalances. These chromosomal alterations have not previously been identified in oncocytoma and may be specific to lacrimal gland oncocytoma. Sequencing of the mitochondrial genome demonstrated multiple alterations of the NADH-ubiquinone oxidoreductase chain 5 (ND5) gene involved in mitochondrial oxidative phosphorylation. This may support the notion of a common genetic background of oncocytic lesions in the lacrimal gland and other anatomical sites.",

author = "Mikkelsen, {Lauge Hjorth} and Simon Andreasen and Melchior, {Linea Cicilie} and Marta Persson and Andersen, {Jeppe Dyrberg} and Vania Pereira and Toft, {Peter Bjerre} and Niels Morling and G{\"o}ran Stenman and Steffen Heegaard",

year = "2017",

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doi = "10.3892/ol.2017.6713",

language = "English",

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T1 - Genomic and immunohistochemical characterisation of a lacrimal gland oncocytoma and review of literature

AU - Mikkelsen, Lauge Hjorth

AU - Andreasen, Simon

AU - Melchior, Linea Cicilie

AU - Persson, Marta

AU - Andersen, Jeppe Dyrberg

AU - Pereira, Vania

AU - Toft, Peter Bjerre

AU - Morling, Niels

AU - Stenman, Göran

AU - Heegaard, Steffen

PY - 2017/10

Y1 - 2017/10

N2 - The aim of the present study was to report the genetic and immunohistochemical profile of a rare case of lacrimal gland oncocytoma. A 20-year-old male underwent magnetic resonance imaging (MRI) due to viral encephalitis. Notably, the MRI revealed a multicystic tumor in the left lacrimal gland. A lateral orbitotomy was performed and the tumor was completely excised. Four months following surgery, the patient was free of symptoms. Histopathologically, the tumor was composed of large, eosinophilic and polyhedral cells with small round nuclei. The tumor cells stained strongly for antimitochondrial antibody MU213-UC, cytokeratin (CK) 5/6, CK 7, CK 17, CK 8/18 and CK 19. The final diagnosis was an oncocytoma of the lacrimal gland without any signs of malignancy. Array-based comparative genomic hybridisation demonstrated a gain of one copy of chromosome 8 and loss of one copy of chromosome 22 as the sole genomic imbalances. These chromosomal alterations have not previously been identified in oncocytoma and may be specific to lacrimal gland oncocytoma. Sequencing of the mitochondrial genome demonstrated multiple alterations of the NADH-ubiquinone oxidoreductase chain 5 (ND5) gene involved in mitochondrial oxidative phosphorylation. This may support the notion of a common genetic background of oncocytic lesions in the lacrimal gland and other anatomical sites.

AB - The aim of the present study was to report the genetic and immunohistochemical profile of a rare case of lacrimal gland oncocytoma. A 20-year-old male underwent magnetic resonance imaging (MRI) due to viral encephalitis. Notably, the MRI revealed a multicystic tumor in the left lacrimal gland. A lateral orbitotomy was performed and the tumor was completely excised. Four months following surgery, the patient was free of symptoms. Histopathologically, the tumor was composed of large, eosinophilic and polyhedral cells with small round nuclei. The tumor cells stained strongly for antimitochondrial antibody MU213-UC, cytokeratin (CK) 5/6, CK 7, CK 17, CK 8/18 and CK 19. The final diagnosis was an oncocytoma of the lacrimal gland without any signs of malignancy. Array-based comparative genomic hybridisation demonstrated a gain of one copy of chromosome 8 and loss of one copy of chromosome 22 as the sole genomic imbalances. These chromosomal alterations have not previously been identified in oncocytoma and may be specific to lacrimal gland oncocytoma. Sequencing of the mitochondrial genome demonstrated multiple alterations of the NADH-ubiquinone oxidoreductase chain 5 (ND5) gene involved in mitochondrial oxidative phosphorylation. This may support the notion of a common genetic background of oncocytic lesions in the lacrimal gland and other anatomical sites.

U2 - 10.3892/ol.2017.6713

DO - 10.3892/ol.2017.6713

M3 - Journal article

C2 - 28943925

SN - 1792-1074

VL - 14

SP - 4176

EP - 4182

JO - Oncology Letters

JF - Oncology Letters

IS - 4

ER -

Genomic and immunohistochemical characterisation of a lacrimal gland oncocytoma and review of literature

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