Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

Agnete Kirkeby; Jenny Nelander; Malin Parmar

doi:10.3389/fncel.2012.00064

Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

Agnete Kirkeby^*, Jenny Nelander, Malin Parmar

^*Corresponding author for this work

Laboratory

22 Citations (Scopus)

Abstract

Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

Original language	English
Journal	Frontiers in Cellular Neuroscience
Issue number	JANUARY 2013
ISSN	1662-5102
DOIs	https://doi.org/10.3389/fncel.2012.00064
Publication status	Published - 3 Jan 2013

Keywords

Brain development
Differentiation
Gsk3
Human embryonic stem cells
Neuronal subtypes
Pluripotent stem cells
Protocol
Regionalization

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3389/fncel.2012.00064

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title = "Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol",

abstract = "Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.",

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T1 - Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

AU - Kirkeby, Agnete

AU - Nelander, Jenny

AU - Parmar, Malin

PY - 2013/1/3

Y1 - 2013/1/3

N2 - Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

AB - Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

KW - Brain development

KW - Differentiation

KW - Gsk3

KW - Human embryonic stem cells

KW - Neuronal subtypes

KW - Pluripotent stem cells

KW - Protocol

KW - Regionalization

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U2 - 10.3389/fncel.2012.00064

DO - 10.3389/fncel.2012.00064

M3 - Journal article

AN - SCOPUS:84872186092

SN - 1662-5102

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

IS - JANUARY 2013

ER -

Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

Abstract

Keywords

UN SDGs

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