Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

Agnete Kirkeby; Jenny Nelander; Malin Parmar

doi:10.3389/fncel.2012.00064

Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

Agnete Kirkeby^*, Jenny Nelander, Malin Parmar

^*Corresponding author af dette arbejde

Laboratory

22 Citationer (Scopus)

Abstract

Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

Originalsprog	Engelsk
Tidsskrift	Frontiers in Cellular Neuroscience
Udgave nummer	JANUARY 2013
ISSN	1662-5102
DOI	https://doi.org/10.3389/fncel.2012.00064
Status	Udgivet - 3 jan. 2013

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title = "Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol",

abstract = "Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.",

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T1 - Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

AU - Kirkeby, Agnete

AU - Nelander, Jenny

AU - Parmar, Malin

PY - 2013/1/3

Y1 - 2013/1/3

N2 - Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

AB - Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

KW - Brain development

KW - Differentiation

KW - Gsk3

KW - Human embryonic stem cells

KW - Neuronal subtypes

KW - Pluripotent stem cells

KW - Protocol

KW - Regionalization

UR - http://www.scopus.com/inward/record.url?scp=84872186092&partnerID=8YFLogxK

U2 - 10.3389/fncel.2012.00064

DO - 10.3389/fncel.2012.00064

M3 - Journal article

AN - SCOPUS:84872186092

SN - 1662-5102

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

IS - JANUARY 2013

ER -

Generating regionalized neuronal cells from pluripotency, a step-by-stepprotocol

Abstract

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