Generalist predator contributions to the control of Tetranychus urticae in strawberry crops documented by PCR-based gut content analysis

Stine Kramer Jacobsen; Lene Sigsgaard; Kristian Hansen; James D Harwood; Eric G Chapman; Mónica A Hurtado; Annette B Jensen

doi:10.1007/s10493-019-00351-x

Generalist predator contributions to the control of Tetranychus urticae in strawberry crops documented by PCR-based gut content analysis

Stine Kramer Jacobsen, Lene Sigsgaard, Kristian Hansen, James D Harwood, Eric G Chapman, Mónica A Hurtado, Annette B Jensen

Section for Organismal Biology

4 Citations (Scopus)

Abstract

The contribution of generalist insect predators to the control of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), an herbivorous pest of many crops, is poorly understood. One of the common insect predators in strawberries is the generalist predatory bug Anthocoris nemorum L. (Hemiptera: Anthocoridae), which has the potential to contribute to the control of pest populations. The feeding of adult A. nemorum on T. urticae was assessed by sampling individuals from an organic strawberry field in Denmark, and using PCR gut content analysis to detect remains of T. urticae within their gut. In the lab, we assessed that the DNA half-life detectability was 21.5 h. Significant numbers of field-collected A. nemorum tested positive for T. urticae prey DNA, with very high numbers in June (62.8%) and August (38.8%). This study presents conclusive evidence that the generalist predator A. nemorum can contribute to the decrease of T. urticae densities in strawberry fields, although the actual contribution in the present study is probably limited because predator populations were relatively low compared to T. urticae. The abundance of T. urticae did not increase significantly during the period of sampling, suggesting that a complex of natural enemies can achieve biological control of T. urticae in protected strawberries.

Original language	English
Journal	Experimental & Applied Acarology
Volume	77
Issue number	2
Pages (from-to)	133–143
ISSN	0168-8162
DOIs	https://doi.org/10.1007/s10493-019-00351-x
Publication status	Published - 1 Feb 2019

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Generalist predator contributions to the control of Tetranychus urticae in strawberry crops documented by PCR-based gut content analysis. / Jacobsen, Stine Kramer ; Sigsgaard, Lene; Hansen, Kristian et al.
In: Experimental & Applied Acarology, Vol. 77, No. 2, 01.02.2019, p. 133–143.

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title = "Generalist predator contributions to the control of Tetranychus urticae in strawberry crops documented by PCR-based gut content analysis",

abstract = "The contribution of generalist insect predators to the control of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), an herbivorous pest of many crops, is poorly understood. One of the common insect predators in strawberries is the generalist predatory bug Anthocoris nemorum L. (Hemiptera: Anthocoridae), which has the potential to contribute to the control of pest populations. The feeding of adult A. nemorum on T. urticae was assessed by sampling individuals from an organic strawberry field in Denmark, and using PCR gut content analysis to detect remains of T. urticae within their gut. In the lab, we assessed that the DNA half-life detectability was 21.5 h. Significant numbers of field-collected A. nemorum tested positive for T. urticae prey DNA, with very high numbers in June (62.8%) and August (38.8%). This study presents conclusive evidence that the generalist predator A. nemorum can contribute to the decrease of T. urticae densities in strawberry fields, although the actual contribution in the present study is probably limited because predator populations were relatively low compared to T. urticae. The abundance of T. urticae did not increase significantly during the period of sampling, suggesting that a complex of natural enemies can achieve biological control of T. urticae in protected strawberries.",

author = "Jacobsen, {Stine Kramer} and Lene Sigsgaard and Kristian Hansen and Harwood, {James D} and Chapman, {Eric G} and Hurtado, {M{\'o}nica A} and Jensen, {Annette B}",

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AU - Harwood, James D

AU - Chapman, Eric G

AU - Hurtado, Mónica A

AU - Jensen, Annette B

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N2 - The contribution of generalist insect predators to the control of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), an herbivorous pest of many crops, is poorly understood. One of the common insect predators in strawberries is the generalist predatory bug Anthocoris nemorum L. (Hemiptera: Anthocoridae), which has the potential to contribute to the control of pest populations. The feeding of adult A. nemorum on T. urticae was assessed by sampling individuals from an organic strawberry field in Denmark, and using PCR gut content analysis to detect remains of T. urticae within their gut. In the lab, we assessed that the DNA half-life detectability was 21.5 h. Significant numbers of field-collected A. nemorum tested positive for T. urticae prey DNA, with very high numbers in June (62.8%) and August (38.8%). This study presents conclusive evidence that the generalist predator A. nemorum can contribute to the decrease of T. urticae densities in strawberry fields, although the actual contribution in the present study is probably limited because predator populations were relatively low compared to T. urticae. The abundance of T. urticae did not increase significantly during the period of sampling, suggesting that a complex of natural enemies can achieve biological control of T. urticae in protected strawberries.

AB - The contribution of generalist insect predators to the control of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), an herbivorous pest of many crops, is poorly understood. One of the common insect predators in strawberries is the generalist predatory bug Anthocoris nemorum L. (Hemiptera: Anthocoridae), which has the potential to contribute to the control of pest populations. The feeding of adult A. nemorum on T. urticae was assessed by sampling individuals from an organic strawberry field in Denmark, and using PCR gut content analysis to detect remains of T. urticae within their gut. In the lab, we assessed that the DNA half-life detectability was 21.5 h. Significant numbers of field-collected A. nemorum tested positive for T. urticae prey DNA, with very high numbers in June (62.8%) and August (38.8%). This study presents conclusive evidence that the generalist predator A. nemorum can contribute to the decrease of T. urticae densities in strawberry fields, although the actual contribution in the present study is probably limited because predator populations were relatively low compared to T. urticae. The abundance of T. urticae did not increase significantly during the period of sampling, suggesting that a complex of natural enemies can achieve biological control of T. urticae in protected strawberries.

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Generalist predator contributions to the control of Tetranychus urticae in strawberry crops documented by PCR-based gut content analysis

Abstract

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