Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration

Rita Cássia Marqueti; Rita de Cássia Marqueti; Katja Maria Heinemeier; João Luiz Quaglioti Durigan; Sérgio Eduardo de Andrade Perez; Peter Schjerling; Michael Kjaer; Hernandes Faustino Carvalho; Heloisa Sobreiro Selistre-de-Araujo

doi:10.1007/s00421-011-2114-x

Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration

Rita Cássia Marqueti, Rita de Cássia Marqueti, Katja Maria Heinemeier, João Luiz Quaglioti Durigan, Sérgio Eduardo de Andrade Perez, Peter Schjerling, Michael Kjaer, Hernandes Faustino Carvalho, Heloisa Sobreiro Selistre-de-Araujo

Damage and Repair

13 Citations (Scopus)

Abstract

The aim of this study was to evaluate the expression of key genes responsible for tendon remodeling of the proximal and distal regions of calcaneal tendon (CT), intermediate and distal region of superficial flexor tendon (SFT) and proximal, intermediate and distal region of deep flexor tendon (DFT) submitted to 7 weeks of jumping water load exercise in combination with AAS administration. Wistar male rats were grouped as follows: sedentary (S), trained (jumping water load exercise) (T), sedentary animals treated with AAS (5 mg/kg, twice a week) and animals treated with AAS and trained (AAST). mRNA levels of COL1A1, COL3A1, TIMP-1, TIMP-2, MMP-2, IGF-IEa, GAPDH, CTGF and TGF-β-1 were evaluated by quantitative PCR. Our main results indicated that mRNA levels alter in different regions in each tendon of sedentary animals. The training did not alter the expression of COL1A1, COL3A, IGF-IEa and MMP-2 genes, while AAS administration or its combination with training reduced their expression. This study indicated that exercise did not alter the expression of collagen and related growth factors in different regions of rat tendon. Moreover, the pattern of gene expression was distinct in the different tendon regions of sedentary animals. Although, the RNA yield levels of CT, SFT and DFT were not distinct in each region, these regions possess not only the structural and biochemical difference, but also divergence in the expression of key genes involved in tendon adaptation.

Original language	English
Journal	Journal of Applied Physiology
Volume	112
Issue number	4
Pages (from-to)	1505-15
Number of pages	11
ISSN	8750-7587
DOIs	https://doi.org/10.1007/s00421-011-2114-x
Publication status	Published - Apr 2012

Keywords

Adaptation, Physiological
Anabolic Agents
Androgens
Animals
Collagen Type I
Collagen Type III
Connective Tissue Growth Factor
Gene Expression Regulation
Glyceraldehyde-3-Phosphate Dehydrogenases
Insulin-Like Growth Factor I
Male
Matrix Metalloproteinase 2
Nandrolone
Physical Exertion
RNA, Messenger
Rats
Rats, Wistar
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Sedentary Lifestyle
Tendons
Time Factors
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Transforming Growth Factor beta1

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10.1007/s00421-011-2114-x

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Marqueti, R. C., Marqueti, R. D. C., Heinemeier, K. M., Durigan, J. L. Q., de Andrade Perez, S. E., Schjerling, P., Kjaer, M., Carvalho, H. F., & Selistre-de-Araujo, H. S. (2012). Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration. Journal of Applied Physiology, 112(4), 1505-15. https://doi.org/10.1007/s00421-011-2114-x

Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration. / Marqueti, Rita Cássia; Marqueti, Rita de Cássia; Heinemeier, Katja Maria et al.
In: Journal of Applied Physiology, Vol. 112, No. 4, 04.2012, p. 1505-15.

Research output: Contribution to journal › Journal article › Research › peer-review

Marqueti, RC, Marqueti, RDC, Heinemeier, KM, Durigan, JLQ, de Andrade Perez, SE, Schjerling, P , Kjaer, M, Carvalho, HF & Selistre-de-Araujo, HS 2012, 'Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration', Journal of Applied Physiology, vol. 112, no. 4, pp. 1505-15. https://doi.org/10.1007/s00421-011-2114-x

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title = "Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration",

abstract = "The aim of this study was to evaluate the expression of key genes responsible for tendon remodeling of the proximal and distal regions of calcaneal tendon (CT), intermediate and distal region of superficial flexor tendon (SFT) and proximal, intermediate and distal region of deep flexor tendon (DFT) submitted to 7 weeks of jumping water load exercise in combination with AAS administration. Wistar male rats were grouped as follows: sedentary (S), trained (jumping water load exercise) (T), sedentary animals treated with AAS (5 mg/kg, twice a week) and animals treated with AAS and trained (AAST). mRNA levels of COL1A1, COL3A1, TIMP-1, TIMP-2, MMP-2, IGF-IEa, GAPDH, CTGF and TGF-β-1 were evaluated by quantitative PCR. Our main results indicated that mRNA levels alter in different regions in each tendon of sedentary animals. The training did not alter the expression of COL1A1, COL3A, IGF-IEa and MMP-2 genes, while AAS administration or its combination with training reduced their expression. This study indicated that exercise did not alter the expression of collagen and related growth factors in different regions of rat tendon. Moreover, the pattern of gene expression was distinct in the different tendon regions of sedentary animals. Although, the RNA yield levels of CT, SFT and DFT were not distinct in each region, these regions possess not only the structural and biochemical difference, but also divergence in the expression of key genes involved in tendon adaptation.",

keywords = "Adaptation, Physiological, Anabolic Agents, Androgens, Animals, Collagen Type I, Collagen Type III, Connective Tissue Growth Factor, Gene Expression Regulation, Glyceraldehyde-3-Phosphate Dehydrogenases, Insulin-Like Growth Factor I, Male, Matrix Metalloproteinase 2, Nandrolone, Physical Exertion, RNA, Messenger, Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Sedentary Lifestyle, Tendons, Time Factors, Tissue Inhibitor of Metalloproteinase-1, Tissue Inhibitor of Metalloproteinase-2, Transforming Growth Factor beta1",

author = "Marqueti, {Rita C{\'a}ssia} and Marqueti, {Rita de C{\'a}ssia} and Heinemeier, {Katja Maria} and Durigan, {Jo{\~a}o Luiz Quaglioti} and {de Andrade Perez}, {S{\'e}rgio Eduardo} and Peter Schjerling and Michael Kjaer and Carvalho, {Hernandes Faustino} and Selistre-de-Araujo, {Heloisa Sobreiro}",

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doi = "10.1007/s00421-011-2114-x",

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AU - Marqueti, Rita Cássia

AU - Marqueti, Rita de Cássia

AU - Heinemeier, Katja Maria

AU - Durigan, João Luiz Quaglioti

AU - de Andrade Perez, Sérgio Eduardo

AU - Schjerling, Peter

AU - Kjaer, Michael

AU - Carvalho, Hernandes Faustino

AU - Selistre-de-Araujo, Heloisa Sobreiro

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N2 - The aim of this study was to evaluate the expression of key genes responsible for tendon remodeling of the proximal and distal regions of calcaneal tendon (CT), intermediate and distal region of superficial flexor tendon (SFT) and proximal, intermediate and distal region of deep flexor tendon (DFT) submitted to 7 weeks of jumping water load exercise in combination with AAS administration. Wistar male rats were grouped as follows: sedentary (S), trained (jumping water load exercise) (T), sedentary animals treated with AAS (5 mg/kg, twice a week) and animals treated with AAS and trained (AAST). mRNA levels of COL1A1, COL3A1, TIMP-1, TIMP-2, MMP-2, IGF-IEa, GAPDH, CTGF and TGF-β-1 were evaluated by quantitative PCR. Our main results indicated that mRNA levels alter in different regions in each tendon of sedentary animals. The training did not alter the expression of COL1A1, COL3A, IGF-IEa and MMP-2 genes, while AAS administration or its combination with training reduced their expression. This study indicated that exercise did not alter the expression of collagen and related growth factors in different regions of rat tendon. Moreover, the pattern of gene expression was distinct in the different tendon regions of sedentary animals. Although, the RNA yield levels of CT, SFT and DFT were not distinct in each region, these regions possess not only the structural and biochemical difference, but also divergence in the expression of key genes involved in tendon adaptation.

AB - The aim of this study was to evaluate the expression of key genes responsible for tendon remodeling of the proximal and distal regions of calcaneal tendon (CT), intermediate and distal region of superficial flexor tendon (SFT) and proximal, intermediate and distal region of deep flexor tendon (DFT) submitted to 7 weeks of jumping water load exercise in combination with AAS administration. Wistar male rats were grouped as follows: sedentary (S), trained (jumping water load exercise) (T), sedentary animals treated with AAS (5 mg/kg, twice a week) and animals treated with AAS and trained (AAST). mRNA levels of COL1A1, COL3A1, TIMP-1, TIMP-2, MMP-2, IGF-IEa, GAPDH, CTGF and TGF-β-1 were evaluated by quantitative PCR. Our main results indicated that mRNA levels alter in different regions in each tendon of sedentary animals. The training did not alter the expression of COL1A1, COL3A, IGF-IEa and MMP-2 genes, while AAS administration or its combination with training reduced their expression. This study indicated that exercise did not alter the expression of collagen and related growth factors in different regions of rat tendon. Moreover, the pattern of gene expression was distinct in the different tendon regions of sedentary animals. Although, the RNA yield levels of CT, SFT and DFT were not distinct in each region, these regions possess not only the structural and biochemical difference, but also divergence in the expression of key genes involved in tendon adaptation.

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KW - Glyceraldehyde-3-Phosphate Dehydrogenases

KW - Insulin-Like Growth Factor I

KW - Male

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KW - Rats, Wistar

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KW - Sedentary Lifestyle

KW - Tendons

KW - Time Factors

KW - Tissue Inhibitor of Metalloproteinase-1

KW - Tissue Inhibitor of Metalloproteinase-2

KW - Transforming Growth Factor beta1

U2 - 10.1007/s00421-011-2114-x

DO - 10.1007/s00421-011-2114-x

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SN - 8750-7587

VL - 112

SP - 1505

EP - 1515

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

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ER -

Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration

Abstract

Keywords

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