Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration

TY - JOUR

T1 - Gene expression in distinct regions of rat tendons in response to jump training combined with anabolic androgenic steroid administration

AU - Marqueti, Rita Cássia

AU - Marqueti, Rita de Cássia

AU - Heinemeier, Katja Maria

AU - Durigan, João Luiz Quaglioti

AU - de Andrade Perez, Sérgio Eduardo

AU - Schjerling, Peter

AU - Kjaer, Michael

AU - Carvalho, Hernandes Faustino

AU - Selistre-de-Araujo, Heloisa Sobreiro

PY - 2012/4

Y1 - 2012/4

N2 - The aim of this study was to evaluate the expression of key genes responsible for tendon remodeling of the proximal and distal regions of calcaneal tendon (CT), intermediate and distal region of superficial flexor tendon (SFT) and proximal, intermediate and distal region of deep flexor tendon (DFT) submitted to 7 weeks of jumping water load exercise in combination with AAS administration. Wistar male rats were grouped as follows: sedentary (S), trained (jumping water load exercise) (T), sedentary animals treated with AAS (5 mg/kg, twice a week) and animals treated with AAS and trained (AAST). mRNA levels of COL1A1, COL3A1, TIMP-1, TIMP-2, MMP-2, IGF-IEa, GAPDH, CTGF and TGF-β-1 were evaluated by quantitative PCR. Our main results indicated that mRNA levels alter in different regions in each tendon of sedentary animals. The training did not alter the expression of COL1A1, COL3A, IGF-IEa and MMP-2 genes, while AAS administration or its combination with training reduced their expression. This study indicated that exercise did not alter the expression of collagen and related growth factors in different regions of rat tendon. Moreover, the pattern of gene expression was distinct in the different tendon regions of sedentary animals. Although, the RNA yield levels of CT, SFT and DFT were not distinct in each region, these regions possess not only the structural and biochemical difference, but also divergence in the expression of key genes involved in tendon adaptation.

AB - The aim of this study was to evaluate the expression of key genes responsible for tendon remodeling of the proximal and distal regions of calcaneal tendon (CT), intermediate and distal region of superficial flexor tendon (SFT) and proximal, intermediate and distal region of deep flexor tendon (DFT) submitted to 7 weeks of jumping water load exercise in combination with AAS administration. Wistar male rats were grouped as follows: sedentary (S), trained (jumping water load exercise) (T), sedentary animals treated with AAS (5 mg/kg, twice a week) and animals treated with AAS and trained (AAST). mRNA levels of COL1A1, COL3A1, TIMP-1, TIMP-2, MMP-2, IGF-IEa, GAPDH, CTGF and TGF-β-1 were evaluated by quantitative PCR. Our main results indicated that mRNA levels alter in different regions in each tendon of sedentary animals. The training did not alter the expression of COL1A1, COL3A, IGF-IEa and MMP-2 genes, while AAS administration or its combination with training reduced their expression. This study indicated that exercise did not alter the expression of collagen and related growth factors in different regions of rat tendon. Moreover, the pattern of gene expression was distinct in the different tendon regions of sedentary animals. Although, the RNA yield levels of CT, SFT and DFT were not distinct in each region, these regions possess not only the structural and biochemical difference, but also divergence in the expression of key genes involved in tendon adaptation.

KW - Adaptation, Physiological

KW - Anabolic Agents

KW - Androgens

KW - Animals

KW - Collagen Type I

KW - Collagen Type III

KW - Connective Tissue Growth Factor

KW - Gene Expression Regulation

KW - Glyceraldehyde-3-Phosphate Dehydrogenases

KW - Insulin-Like Growth Factor I

KW - Male

KW - Matrix Metalloproteinase 2

KW - Nandrolone

KW - Physical Exertion

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sedentary Lifestyle

KW - Tendons

KW - Time Factors

KW - Tissue Inhibitor of Metalloproteinase-1

KW - Tissue Inhibitor of Metalloproteinase-2

KW - Transforming Growth Factor beta1

U2 - 10.1007/s00421-011-2114-x

DO - 10.1007/s00421-011-2114-x

M3 - Journal article

C2 - 21842416

SN - 8750-7587

VL - 112

SP - 1505

EP - 1515

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 4

ER -