Gamma-aminobutyric acid agonist-induced alterations in the ultrastructure of cultured cerebellar granule cells is restricted to early development

Gert Helge Hansen; B Belhage; A Schousboe; E Meier

Gamma-aminobutyric acid agonist-induced alterations in the ultrastructure of cultured cerebellar granule cells is restricted to early development

Gert Helge Hansen, B Belhage, A Schousboe, E Meier

Department of Cellular and Molecular Medicine

19 Citations (Scopus)

Abstract

The effect of 4,5,6,7-tetrahydroisoxazolo[5,4-c]-pyridin-3-ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 microM) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7-day-old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14-day-old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.

Original language	English
Journal	Journal of Neurochemistry
Volume	51
Issue number	1
Pages (from-to)	243-5
Number of pages	3
ISSN	0022-3042
Publication status	Published - 1988

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title = "Gamma-aminobutyric acid agonist-induced alterations in the ultrastructure of cultured cerebellar granule cells is restricted to early development",

abstract = "The effect of 4,5,6,7-tetrahydroisoxazolo[5,4-c]-pyridin-3-ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 microM) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7-day-old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14-day-old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.",

author = "Hansen, {Gert Helge} and B Belhage and A Schousboe and E Meier",

note = "Keywords: Animals; Cells, Cultured; Cerebellum; Cytoplasm; Endoplasmic Reticulum; Golgi Apparatus; Isoxazoles; Microscopy, Electron; Oxazoles; Rats; Rats, Inbred Strains; gamma-Aminobutyric Acid",

year = "1988",

language = "English",

volume = "51",

pages = "243--5",

journal = "Journal of Neurochemistry",

issn = "0022-3042",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Gamma-aminobutyric acid agonist-induced alterations in the ultrastructure of cultured cerebellar granule cells is restricted to early development

AU - Hansen, Gert Helge

AU - Belhage, B

AU - Schousboe, A

AU - Meier, E

N1 - Keywords: Animals; Cells, Cultured; Cerebellum; Cytoplasm; Endoplasmic Reticulum; Golgi Apparatus; Isoxazoles; Microscopy, Electron; Oxazoles; Rats; Rats, Inbred Strains; gamma-Aminobutyric Acid

PY - 1988

Y1 - 1988

N2 - The effect of 4,5,6,7-tetrahydroisoxazolo[5,4-c]-pyridin-3-ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 microM) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7-day-old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14-day-old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.

AB - The effect of 4,5,6,7-tetrahydroisoxazolo[5,4-c]-pyridin-3-ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 microM) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7-day-old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14-day-old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.

M3 - Journal article

C2 - 3379406

SN - 0022-3042

VL - 51

SP - 243

EP - 245

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

IS - 1

ER -

Gamma-aminobutyric acid agonist-induced alterations in the ultrastructure of cultured cerebellar granule cells is restricted to early development

Abstract

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