TY - JOUR
T1 - Gamma-aminobutyric acid agonist-induced alterations in the ultrastructure of cultured cerebellar granule cells is restricted to early development
AU - Hansen, Gert Helge
AU - Belhage, B
AU - Schousboe, A
AU - Meier, E
N1 - Keywords: Animals; Cells, Cultured; Cerebellum; Cytoplasm; Endoplasmic Reticulum; Golgi Apparatus; Isoxazoles; Microscopy, Electron; Oxazoles; Rats; Rats, Inbred Strains; gamma-Aminobutyric Acid
PY - 1988
Y1 - 1988
N2 - The effect of 4,5,6,7-tetrahydroisoxazolo[5,4-c]-pyridin-3-ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 microM) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7-day-old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14-day-old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.
AB - The effect of 4,5,6,7-tetrahydroisoxazolo[5,4-c]-pyridin-3-ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 microM) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7-day-old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14-day-old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.
M3 - Journal article
C2 - 3379406
SN - 0022-3042
VL - 51
SP - 243
EP - 245
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 1
ER -