Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device

Nikolaj A. Feidenhans; Thomas Glasdam Jensen; Josiane P. Lafleur; Jörg P. Kutter

Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device

Nikolaj A. Feidenhans, Thomas Glasdam Jensen, Josiane P. Lafleur, Jörg P. Kutter^*

^*Corresponding author for this work

1 Citation (Scopus)

Abstract

We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.

Original language	English
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Number of pages	3
Volume	2
Publisher	Chemical and Biological Microsystems Society
Publication date	2013
Pages	745-747
ISBN (Print)	9781632666246
Publication status	Published - 2013

Keywords

Biomolecule immobilization
Optofluidic chip
Thiol-ene polymers

Cite this

Feidenhans, N. A., Jensen, T. G., Lafleur, J. P., & Kutter, J. P. (2013). Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 2, pp. 745-747). Chemical and Biological Microsystems Society.

Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. / Feidenhans, Nikolaj A.; Jensen, Thomas Glasdam; Lafleur, Josiane P. et al.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. p. 745-747.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Feidenhans, Nikolaj A. ; Jensen, Thomas Glasdam ; Lafleur, Josiane P. et al. / Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. pp. 745-747

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abstract = "We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.",

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AU - Feidenhans, Nikolaj A.

AU - Jensen, Thomas Glasdam

AU - Lafleur, Josiane P.

AU - Kutter, Jörg P.

PY - 2013

Y1 - 2013

N2 - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.

AB - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.

KW - Biomolecule immobilization

KW - Optofluidic chip

KW - Thiol-ene polymers

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M3 - Article in proceedings

AN - SCOPUS:84907330820

SN - 9781632666246

VL - 2

SP - 745

EP - 747

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

ER -

Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device

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Keywords

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