TY - GEN
T1 - Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device
AU - Feidenhans, Nikolaj A.
AU - Jensen, Thomas Glasdam
AU - Lafleur, Josiane P.
AU - Kutter, Jörg P.
PY - 2013
Y1 - 2013
N2 - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.
AB - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.
KW - Biomolecule immobilization
KW - Optofluidic chip
KW - Thiol-ene polymers
UR - http://www.scopus.com/inward/record.url?scp=84907330820&partnerID=8YFLogxK
M3 - Article in proceedings
AN - SCOPUS:84907330820
SN - 9781632666246
VL - 2
SP - 745
EP - 747
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
ER -