Functional properties of human neuronal Kv11 channels

Karoline Einarsen; Kirstine Calloe; Morten Grunnet; Søren-Peter Olesen; Nicole Schmitt

doi:10.1007/s00424-009-0651-5

Functional properties of human neuronal Kv11 channels

Karoline Einarsen, Kirstine Calloe, Morten Grunnet, Søren-Peter Olesen, Nicole Schmitt

7 Citations (Scopus)

Abstract

Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present a new splice variant that is present in human brain as demonstrated by reverse transcription PCR. Heterologous expression in Xenopus laevis oocytes revealed a 30-mV shift in the voltage dependence of activation to more depolarized potentials and slower activation together with faster deactivation kinetics compared to hKv11.1. Further, we have cloned and electrophysiologically characterized two splice variants of hKv11.3. When expressed in X. laevis oocytes, both isoform 1 and isoform 2 elicited robust currents with a striking transient current component caused by delayed inactivation. The different current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability.

Original language	English
Journal	Pflügers Archiv: European Journal of Physiology
Volume	458
Issue number	4
Pages (from-to)	689-700
Number of pages	11
ISSN	0031-6768
DOIs	https://doi.org/10.1007/s00424-009-0651-5
Publication status	Published - 2009

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title = "Functional properties of human neuronal Kv11 channels",

abstract = "Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present a new splice variant that is present in human brain as demonstrated by reverse transcription PCR. Heterologous expression in Xenopus laevis oocytes revealed a 30-mV shift in the voltage dependence of activation to more depolarized potentials and slower activation together with faster deactivation kinetics compared to hKv11.1. Further, we have cloned and electrophysiologically characterized two splice variants of hKv11.3. When expressed in X. laevis oocytes, both isoform 1 and isoform 2 elicited robust currents with a striking transient current component caused by delayed inactivation. The different current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability.",

author = "Karoline Einarsen and Kirstine Calloe and Morten Grunnet and S{\o}ren-Peter Olesen and Nicole Schmitt",

note = "Keywords: Action Potentials; Animals; Cells, Cultured; Ion Channel Gating; Membrane Potentials; Neurons; Oocytes; Potassium Channels; Xenopus laevis",

year = "2009",

doi = "10.1007/s00424-009-0651-5",

language = "English",

volume = "458",

pages = "689--700",

journal = "Pfl{\"u}gers Archiv - European Journal of Physiology",

issn = "0031-6768",

publisher = "Springer",

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TY - JOUR

T1 - Functional properties of human neuronal Kv11 channels

AU - Einarsen, Karoline

AU - Calloe, Kirstine

AU - Grunnet, Morten

AU - Olesen, Søren-Peter

AU - Schmitt, Nicole

N1 - Keywords: Action Potentials; Animals; Cells, Cultured; Ion Channel Gating; Membrane Potentials; Neurons; Oocytes; Potassium Channels; Xenopus laevis

PY - 2009

Y1 - 2009

N2 - Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present a new splice variant that is present in human brain as demonstrated by reverse transcription PCR. Heterologous expression in Xenopus laevis oocytes revealed a 30-mV shift in the voltage dependence of activation to more depolarized potentials and slower activation together with faster deactivation kinetics compared to hKv11.1. Further, we have cloned and electrophysiologically characterized two splice variants of hKv11.3. When expressed in X. laevis oocytes, both isoform 1 and isoform 2 elicited robust currents with a striking transient current component caused by delayed inactivation. The different current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability.

AB - Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present a new splice variant that is present in human brain as demonstrated by reverse transcription PCR. Heterologous expression in Xenopus laevis oocytes revealed a 30-mV shift in the voltage dependence of activation to more depolarized potentials and slower activation together with faster deactivation kinetics compared to hKv11.1. Further, we have cloned and electrophysiologically characterized two splice variants of hKv11.3. When expressed in X. laevis oocytes, both isoform 1 and isoform 2 elicited robust currents with a striking transient current component caused by delayed inactivation. The different current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability.

U2 - 10.1007/s00424-009-0651-5

DO - 10.1007/s00424-009-0651-5

M3 - Journal article

C2 - 19319565

SN - 0031-6768

VL - 458

SP - 689

EP - 700

JO - Pflügers Archiv - European Journal of Physiology

JF - Pflügers Archiv - European Journal of Physiology

IS - 4

ER -

Functional properties of human neuronal Kv11 channels

Abstract

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