FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis

L Rebekka Ryder; Else Marie Bartels; Anders Woetmann; Hans Ole Madsen; Niels Ødum; Henning Bliddal; Bente Danneskiold-Samsøe; Søren Ribel-Madsen; Lars P Ryder

doi:10.1111/j.1600-0463.2011.02848.x

FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis

L Rebekka Ryder, Else Marie Bartels, Anders Woetmann, Hans Ole Madsen, Niels Ødum, Henning Bliddal, Bente Danneskiold-Samsøe, Søren Ribel-Madsen, Lars P Ryder

Cell Biology and Physiology

16 Citations (Scopus)

Abstract

Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.

Original language	English
Journal	APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Volume	120
Issue number	5
Pages (from-to)	387-396
Number of pages	10
ISSN	0903-4641
DOIs	https://doi.org/10.1111/j.1600-0463.2011.02848.x
Publication status	Published - May 2012

Keywords

Adult
Aged
Aged, 80 and over
Alternative Splicing
Arthritis, Psoriatic
Arthritis, Rheumatoid
CD4-Positive T-Lymphocytes
Female
Forkhead Transcription Factors
Humans
Male
Middle Aged
Protein Isoforms
RNA, Messenger
Real-Time Polymerase Chain Reaction
Statistics, Nonparametric
Synovial Fluid
Young Adult

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Ryder, L. R., Bartels, E. M., Woetmann, A., Madsen, H. O., Ødum, N., Bliddal, H., Danneskiold-Samsøe, B., Ribel-Madsen, S., & Ryder, L. P. (2012). FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 120(5), 387-396. https://doi.org/10.1111/j.1600-0463.2011.02848.x

Ryder, LR, Bartels, EM, Woetmann, A, Madsen, HO, Ødum, N, Bliddal, H, Danneskiold-Samsøe, B, Ribel-Madsen, S & Ryder, LP 2012, 'FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis', APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, vol. 120, no. 5, pp. 387-396. https://doi.org/10.1111/j.1600-0463.2011.02848.x

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abstract = "Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.",

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T1 - FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis

AU - Ryder, L Rebekka

AU - Bartels, Else Marie

AU - Woetmann, Anders

AU - Madsen, Hans Ole

AU - Ødum, Niels

AU - Bliddal, Henning

AU - Danneskiold-Samsøe, Bente

AU - Ribel-Madsen, Søren

AU - Ryder, Lars P

PY - 2012/5

Y1 - 2012/5

N2 - Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.

AB - Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Alternative Splicing

KW - Arthritis, Psoriatic

KW - Arthritis, Rheumatoid

KW - CD4-Positive T-Lymphocytes

KW - Female

KW - Forkhead Transcription Factors

KW - Humans

KW - Male

KW - Middle Aged

KW - Protein Isoforms

KW - RNA, Messenger

KW - Real-Time Polymerase Chain Reaction

KW - Statistics, Nonparametric

KW - Synovial Fluid

KW - Young Adult

U2 - 10.1111/j.1600-0463.2011.02848.x

DO - 10.1111/j.1600-0463.2011.02848.x

M3 - Journal article

C2 - 22515293

SN - 0903-465X

VL - 120

SP - 387

EP - 396

JO - APMIS. Supplementum

JF - APMIS. Supplementum

IS - 5

ER -

FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis

Abstract

Keywords

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