Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism

TY - JOUR

T1 - Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism

AU - Matthiesen, Connie Frank

AU - Casañas, Maria Arantzazu Aguinaga

AU - Tauson, Anne-Helene

PY - 2014/3

Y1 - 2014/3

N2 - The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F0- and F1-generation dams and in F1-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F1 foetuses exposed to low protein during foetal life than F0 dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial β-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.

AB - The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F0- and F1-generation dams and in F1-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F1 foetuses exposed to low protein during foetal life than F0 dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial β-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.

U2 - 10.1080/1745039x.2014.889506

DO - 10.1080/1745039x.2014.889506

M3 - Journal article

C2 - 24646153

SN - 1745-039X

VL - 68

SP - 159

EP - 169

JO - Archives of Animal Nutrition

JF - Archives of Animal Nutrition

IS - 2

ER -