Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism

Connie Frank Matthiesen; Maria Arantzazu Aguinaga  Casañas; Anne-Helene Tauson

doi:10.1080/1745039x.2014.889506

Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism

Connie Frank Matthiesen, Maria Arantzazu Aguinaga Casañas, Anne-Helene Tauson

2 Citationer (Scopus)

Abstract

The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F₀- and F₁-generation dams and in F₁-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F₁ foetuses exposed to low protein during foetal life than F₀ dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial β-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.

Originalsprog	Engelsk
Tidsskrift	Archives of Animal Nutrition
Vol/bind	68
Udgave nummer	2
Sider (fra-til)	159-169
Antal sider	11
ISSN	1745-039X
DOI	https://doi.org/10.1080/1745039x.2014.889506
Status	Udgivet - mar. 2014

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10.1080/1745039x.2014.889506

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Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism. / Matthiesen, Connie Frank; Casañas, Maria Arantzazu Aguinaga ; Tauson, Anne-Helene.

I: Archives of Animal Nutrition, Bind 68, Nr. 2, 03.2014, s. 159-169.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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abstract = "The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F0- and F1-generation dams and in F1-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F1 foetuses exposed to low protein during foetal life than F0 dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial β-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.",

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AU - Tauson, Anne-Helene

PY - 2014/3

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N2 - The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F0- and F1-generation dams and in F1-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F1 foetuses exposed to low protein during foetal life than F0 dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial β-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.

AB - The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F0- and F1-generation dams and in F1-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F1 foetuses exposed to low protein during foetal life than F0 dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial β-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.

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SN - 1745-039X

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JO - Archives of Animal Nutrition

JF - Archives of Animal Nutrition

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Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism

Abstract

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