Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Carolin Seuring; Kartik Ayyer; Eleftheria Filippaki; Miriam Barthelmess; Jean-Nicolas Longchamp; Philippe Ringler; Tommaso Pardini; David H Wojtas; Matthew A Coleman; Katerina Dörner; Silje Fuglerud; Greger Hammarin; Birgit Habenstein; Annette E Langkilde; Antoine Loquet; Alke Meents; Roland Riek; Henning Stahlberg; Sébastien Boutet; Mark S Hunter; Jason Koglin; Mengning Liang; Helen M Ginn; Rick P Millane; Matthias Frank; Anton Barty; Henry N Chapman

doi:10.1038/s41467-018-04116-9

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Carolin Seuring, Kartik Ayyer, Eleftheria Filippaki, Miriam Barthelmess, Jean-Nicolas Longchamp, Philippe Ringler, Tommaso Pardini, David H Wojtas, Matthew A Coleman, Katerina Dörner, Silje Fuglerud, Greger Hammarin, Birgit Habenstein, Annette E Langkilde, Antoine Loquet, Alke Meents, Roland Riek, Henning Stahlberg, Sébastien Boutet, Mark S HunterJason Koglin, Mengning Liang, Helen M Ginn, Rick P Millane, Matthias Frank, Anton Barty, Henry N Chapman

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Abstract

Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

Original language	English
Article number	1836
Journal	Nature Communications
Volume	9
Issue number	1
Number of pages	10
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-018-04116-9
Publication status	Published - 1 Dec 2018

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Seuring, C., Ayyer, K., Filippaki, E., Barthelmess, M., Longchamp, J-N., Ringler, P., Pardini, T., Wojtas, D. H., Coleman, M. A., Dörner, K., Fuglerud, S., Hammarin, G., Habenstein, B., Langkilde, A. E., Loquet, A., Meents, A., Riek, R., Stahlberg, H., Boutet, S., ... Chapman, H. N. (2018). Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. Nature Communications, 9(1), [1836]. https://doi.org/10.1038/s41467-018-04116-9

Seuring, C, Ayyer, K, Filippaki, E, Barthelmess, M, Longchamp, J-N, Ringler, P, Pardini, T, Wojtas, DH, Coleman, MA, Dörner, K, Fuglerud, S, Hammarin, G, Habenstein, B, Langkilde, AE, Loquet, A, Meents, A, Riek, R, Stahlberg, H, Boutet, S, Hunter, MS, Koglin, J, Liang, M, Ginn, HM, Millane, RP, Frank, M, Barty, A & Chapman, HN 2018, 'Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene', Nature Communications, vol. 9, no. 1, 1836. https://doi.org/10.1038/s41467-018-04116-9

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title = "Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene",

abstract = "Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 {\AA} and 2.4 {\AA} resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.",

author = "Carolin Seuring and Kartik Ayyer and Eleftheria Filippaki and Miriam Barthelmess and Jean-Nicolas Longchamp and Philippe Ringler and Tommaso Pardini and Wojtas, {David H} and Coleman, {Matthew A} and Katerina D{\"o}rner and Silje Fuglerud and Greger Hammarin and Birgit Habenstein and Langkilde, {Annette E} and Antoine Loquet and Alke Meents and Roland Riek and Henning Stahlberg and S{\'e}bastien Boutet and Hunter, {Mark S} and Jason Koglin and Mengning Liang and Ginn, {Helen M} and Millane, {Rick P} and Matthias Frank and Anton Barty and Chapman, {Henry N}",

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AU - Seuring, Carolin

AU - Ayyer, Kartik

AU - Filippaki, Eleftheria

AU - Barthelmess, Miriam

AU - Longchamp, Jean-Nicolas

AU - Ringler, Philippe

AU - Pardini, Tommaso

AU - Wojtas, David H

AU - Coleman, Matthew A

AU - Dörner, Katerina

AU - Fuglerud, Silje

AU - Hammarin, Greger

AU - Habenstein, Birgit

AU - Langkilde, Annette E

AU - Loquet, Antoine

AU - Meents, Alke

AU - Riek, Roland

AU - Stahlberg, Henning

AU - Boutet, Sébastien

AU - Hunter, Mark S

AU - Koglin, Jason

AU - Liang, Mengning

AU - Ginn, Helen M

AU - Millane, Rick P

AU - Frank, Matthias

AU - Barty, Anton

AU - Chapman, Henry N

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

AB - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

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DO - 10.1038/s41467-018-04116-9

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VL - 9

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 1836

ER -

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

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