Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

Robert A Jacobs; Víctor Díaz; Lavinia Soldini; Thomas Haider; Martin Thomassen; Nikolai B Nordsborg; Max Gassmann; Carsten Lundby

doi:10.1093/gerona/gls335

Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

Robert A Jacobs, Víctor Díaz, Lavinia Soldini, Thomas Haider, Martin Thomassen, Nikolai B Nordsborg, Max Gassmann, Carsten Lundby

The August Krogh Section for Human Physiology

17 Citations (Scopus)

Abstract

The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type.

Original language	English
Journal	Journals of Gerontology. Series A: Biological Sciences & Medical Sciences
Volume	68
Issue number	9
Pages (from-to)	1010-1022
Number of pages	12
ISSN	1079-5006
DOIs	https://doi.org/10.1093/gerona/gls335
Publication status	Published - Sept 2013

Access to Document

10.1093/gerona/gls335

Cite this

Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function. / Jacobs, Robert A; Díaz, Víctor; Soldini, Lavinia et al.

In: Journals of Gerontology. Series A: Biological Sciences & Medical Sciences, Vol. 68, No. 9, 09.2013, p. 1010-1022.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{d7b32f1643274cac898887a3cf76ee53,

title = "Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function",

abstract = "The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type.",

author = "Jacobs, {Robert A} and V{\'i}ctor D{\'i}az and Lavinia Soldini and Thomas Haider and Martin Thomassen and Nordsborg, {Nikolai B} and Max Gassmann and Carsten Lundby",

note = "CURIS 2013 NEXS 100",

year = "2013",

month = sep,

doi = "10.1093/gerona/gls335",

language = "English",

volume = "68",

pages = "1010--1022",

journal = "Journals of Gerontology. Series A: Biological Sciences & Medical Sciences",

issn = "1079-5006",

publisher = "Oxford University Press",

number = "9",

}

TY - JOUR

T1 - Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

AU - Jacobs, Robert A

AU - Díaz, Víctor

AU - Soldini, Lavinia

AU - Haider, Thomas

AU - Thomassen, Martin

AU - Nordsborg, Nikolai B

AU - Gassmann, Max

AU - Lundby, Carsten

N1 - CURIS 2013 NEXS 100

PY - 2013/9

Y1 - 2013/9

N2 - The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type.

AB - The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type.

U2 - 10.1093/gerona/gls335

DO - 10.1093/gerona/gls335

M3 - Journal article

C2 - 23371970

SN - 1079-5006

VL - 68

SP - 1010

EP - 1022

JO - Journals of Gerontology. Series A: Biological Sciences & Medical Sciences

JF - Journals of Gerontology. Series A: Biological Sciences & Medical Sciences

IS - 9

ER -

Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

Abstract

Access to Document

Fingerprint

Cite this