Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

TY - JOUR

T1 - Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

AU - Jacobs, Robert A

AU - Díaz, Víctor

AU - Soldini, Lavinia

AU - Haider, Thomas

AU - Thomassen, Martin

AU - Nordsborg, Nikolai B

AU - Gassmann, Max

AU - Lundby, Carsten

N1 - CURIS 2013 NEXS 100

PY - 2013/9

Y1 - 2013/9

N2 - The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type.

AB - The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type.

U2 - 10.1093/gerona/gls335

DO - 10.1093/gerona/gls335

M3 - Journal article

C2 - 23371970

SN - 1079-5006

VL - 68

SP - 1010

EP - 1022

JO - Journals of Gerontology. Series A: Biological Sciences & Medical Sciences

JF - Journals of Gerontology. Series A: Biological Sciences & Medical Sciences

IS - 9

ER -