Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms

Carmen  Espinosa-Gongora; J. Larsen; Arshnee Moodley; Jens Peter Nielsen; R. L. Skov; M. Andreasen; Luca Guardabassi

doi:10.1017/s0950268811002391

Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms

Carmen Espinosa-Gongora, J. Larsen, Arshnee Moodley, Jens Peter Nielsen, R. L. Skov, M. Andreasen, Luca Guardabassi

10 Citations (Scopus)

Abstract

The objective of this study was to investigate the genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 using pulsed-field gel electrophoresis (PFGE). Dust and pigs at five age groups were sampled in six Danish MRSA-positive pig farms. MRSA CC398 was isolated from 284 of the 391 samples tested, including 230 (74%) animal and 54 (68%) environmental samples. PFGE analysis of a subset of 48 isolates, including the six strains previously isolated from farm workers, revealed the existence of farm-specific pulsotypes. With a single exception, human, environmental and porcine isolates originating from the same farm clustered together in the PFGE cluster analysis, indicating that spread of MRSA CC398 in Danish pig farms is mainly due to clonal dissemination of farm-specific lineages that can be discriminated by PFGE. This finding has important implications for planning future epidemiological studies investigating the spread of CC398 in pig farming.

Original language	English
Journal	Epidemiology and Infection
Volume	140
Issue number	10
Pages (from-to)	1794-1799
Number of pages	6
ISSN	0950-2688
DOIs	https://doi.org/10.1017/s0950268811002391
Publication status	Published - Oct 2012

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Former LIFE faculty

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@article{4a55f539dc3e43afa5ad90d134c55b23,

title = "Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms",

abstract = "The objective of this study was to investigate the genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 using pulsed-field gel electrophoresis (PFGE). Dust and pigs at five age groups were sampled in six Danish MRSA-positive pig farms. MRSA CC398 was isolated from 284 of the 391 samples tested, including 230 (74%) animal and 54 (68%) environmental samples. PFGE analysis of a subset of 48 isolates, including the six strains previously isolated from farm workers, revealed the existence of farm-specific pulsotypes. With a single exception, human, environmental and porcine isolates originating from the same farm clustered together in the PFGE cluster analysis, indicating that spread of MRSA CC398 in Danish pig farms is mainly due to clonal dissemination of farm-specific lineages that can be discriminated by PFGE. This finding has important implications for planning future epidemiological studies investigating the spread of CC398 in pig farming.",

keywords = "Former LIFE faculty, Antibiotic resistance, MRSA, Swine, zoonoses",

author = "Carmen Espinosa-Gongora and J. Larsen and Arshnee Moodley and Nielsen, {Jens Peter} and Skov, {R. L.} and M. Andreasen and Luca Guardabassi",

year = "2012",

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T1 - Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms

AU - Espinosa-Gongora, Carmen

AU - Larsen, J.

AU - Moodley, Arshnee

AU - Nielsen, Jens Peter

AU - Skov, R. L.

AU - Andreasen, M.

AU - Guardabassi, Luca

PY - 2012/10

Y1 - 2012/10

N2 - The objective of this study was to investigate the genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 using pulsed-field gel electrophoresis (PFGE). Dust and pigs at five age groups were sampled in six Danish MRSA-positive pig farms. MRSA CC398 was isolated from 284 of the 391 samples tested, including 230 (74%) animal and 54 (68%) environmental samples. PFGE analysis of a subset of 48 isolates, including the six strains previously isolated from farm workers, revealed the existence of farm-specific pulsotypes. With a single exception, human, environmental and porcine isolates originating from the same farm clustered together in the PFGE cluster analysis, indicating that spread of MRSA CC398 in Danish pig farms is mainly due to clonal dissemination of farm-specific lineages that can be discriminated by PFGE. This finding has important implications for planning future epidemiological studies investigating the spread of CC398 in pig farming.

AB - The objective of this study was to investigate the genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 using pulsed-field gel electrophoresis (PFGE). Dust and pigs at five age groups were sampled in six Danish MRSA-positive pig farms. MRSA CC398 was isolated from 284 of the 391 samples tested, including 230 (74%) animal and 54 (68%) environmental samples. PFGE analysis of a subset of 48 isolates, including the six strains previously isolated from farm workers, revealed the existence of farm-specific pulsotypes. With a single exception, human, environmental and porcine isolates originating from the same farm clustered together in the PFGE cluster analysis, indicating that spread of MRSA CC398 in Danish pig farms is mainly due to clonal dissemination of farm-specific lineages that can be discriminated by PFGE. This finding has important implications for planning future epidemiological studies investigating the spread of CC398 in pig farming.

KW - Former LIFE faculty

KW - Antibiotic resistance

KW - MRSA

KW - Swine

KW - zoonoses

U2 - 10.1017/s0950268811002391

DO - 10.1017/s0950268811002391

M3 - Journal article

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SN - 0950-2688

VL - 140

SP - 1794

EP - 1799

JO - Epidemiology and Infection

JF - Epidemiology and Infection

IS - 10

ER -

Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms

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Keywords

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