Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1

Nikolaj Kulahin; Christina Kasper; Ole Kristensen; Jette Sandholm Kastrup; Vladimir Berezin; Elisabeth Bock; Michael Gajhede

doi:10.1107/S1744309105026783

Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1

Nikolaj Kulahin, Christina Kasper, Ole Kristensen, Jette Sandholm Kastrup, Vladimir Berezin, Elisabeth Bock, Michael Gajhede

Department of Neuroscience

3 Citations (Scopus)

Abstract

Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.

Original language	English
Journal	Acta Crystallographica. Section F : Structural Biology and Crystallization Communications
Volume	61
Issue number	Pt 9
Pages (from-to)	858-60
Number of pages	2
ISSN	1744-3091
DOIs	https://doi.org/10.1107/S1744309105026783
Publication status	Published - 2005

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Kulahin, N., Kasper, C., Kristensen, O., Kastrup, J. S., Berezin, V., Bock, E., & Gajhede, M. (2005). Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1. Acta Crystallographica. Section F : Structural Biology and Crystallization Communications, 61(Pt 9), 858-60. https://doi.org/10.1107/S1744309105026783

Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1. / Kulahin, Nikolaj; Kasper, Christina; Kristensen, Ole et al.

In: Acta Crystallographica. Section F : Structural Biology and Crystallization Communications, Vol. 61, No. Pt 9, 2005, p. 858-60.

Research output: Contribution to journal › Journal article › Research › peer-review

Kulahin, N, Kasper, C, Kristensen, O, Kastrup, JS, Berezin, V, Bock, E & Gajhede, M 2005, 'Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1', Acta Crystallographica. Section F : Structural Biology and Crystallization Communications, vol. 61, no. Pt 9, pp. 858-60. https://doi.org/10.1107/S1744309105026783

Kulahin N, Kasper C, Kristensen O, Kastrup JS, Berezin V, Bock E et al. Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1. Acta Crystallographica. Section F : Structural Biology and Crystallization Communications. 2005;61(Pt 9):858-60. doi: 10.1107/S1744309105026783

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title = "Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1",

abstract = "Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.",

author = "Nikolaj Kulahin and Christina Kasper and Ole Kristensen and Kastrup, {Jette Sandholm} and Vladimir Berezin and Elisabeth Bock and Michael Gajhede",

note = "Keywords: Animals; Crystallization; Crystallography, X-Ray; Fibronectins; Gene Expression; Immunoglobulins; Mice; Neural Cell Adhesion Molecule L1",

year = "2005",

doi = "10.1107/S1744309105026783",

language = "English",

volume = "61",

pages = "858--60",

journal = "Acta Crystallographica Section F: Structural Biology Communications",

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T1 - Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1

AU - Kulahin, Nikolaj

AU - Kasper, Christina

AU - Kristensen, Ole

AU - Kastrup, Jette Sandholm

AU - Berezin, Vladimir

AU - Bock, Elisabeth

AU - Gajhede, Michael

N1 - Keywords: Animals; Crystallization; Crystallography, X-Ray; Fibronectins; Gene Expression; Immunoglobulins; Mice; Neural Cell Adhesion Molecule L1

PY - 2005

Y1 - 2005

N2 - Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.

AB - Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.

U2 - 10.1107/S1744309105026783

DO - 10.1107/S1744309105026783

M3 - Journal article

C2 - 16511179

SN - 2053-230X

VL - 61

SP - 858

EP - 860

JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

IS - Pt 9

ER -

Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1

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