Expression and location of mRNAs encoding multiple forms of secretory phospholipase A2 in the rat retina

TY - JOUR

T1 - Expression and location of mRNAs encoding multiple forms of secretory phospholipase A2 in the rat retina

AU - Kolko, Miriam

AU - Christoffersen, Nanna R

AU - Barreiro, Sebastian G

AU - Bazan, Nicolas G

AU - Kolko, Miriam

N1 - Copyright 2004 Wiley-Liss, Inc.

PY - 2004/8/15

Y1 - 2004/8/15

N2 - Low-molecular-weight secretory phospholipases A(2) (sPLA(2)s) are a subgroup of PLA(2)s, which are secreted, bind to receptors, and may act as intercellular signaling modulators. At least 10 different groups have been characterized in mammals, and there is expanding evidence of the significance of sPLA(2)s in neuronal signaling and survival [Kolko et al. (1996) J. Biol. Chem. 271: 32722-32728]. To date, no retinal sPLA(2)s have been cloned or characterized. We evaluated the existence and abundance of sPLA(2) subtypes in rat retina and explored their possible involvement in light-induced retinal damage. We designed primers to identify the sPLA(2)s in rat retina, based on known sequences of sPLA(2)-specific mRNAs in other tissues. RNA was isolated from rat retina, and cDNA was produced and used for PCR cloning to identify the novel subtypes of sPLA(2). Our study revealed the presence of mRNAs encoding sPLA(2)-IB, -X, -V, -IIE, -IIA, and -IIF in the retina, and quantification by real-time PCR revealed different abundances of the sPLA(2)s. We showed a time-dependent gene induction of sPLA(2)-X, -IB, and -V in light-induced retinal damage. We further explored the location of sPLA(2)-IB by in situ hybridization and immunohistochemistry. This study is the first to reveal the presence, abundance, and induction of mRNAs encoding sPLA(2)s in rat retina. We suggest that these enzymes are themselves intercellular signaling modulators of retinal cell function and perhaps also of retinal degeneration.

AB - Low-molecular-weight secretory phospholipases A(2) (sPLA(2)s) are a subgroup of PLA(2)s, which are secreted, bind to receptors, and may act as intercellular signaling modulators. At least 10 different groups have been characterized in mammals, and there is expanding evidence of the significance of sPLA(2)s in neuronal signaling and survival [Kolko et al. (1996) J. Biol. Chem. 271: 32722-32728]. To date, no retinal sPLA(2)s have been cloned or characterized. We evaluated the existence and abundance of sPLA(2) subtypes in rat retina and explored their possible involvement in light-induced retinal damage. We designed primers to identify the sPLA(2)s in rat retina, based on known sequences of sPLA(2)-specific mRNAs in other tissues. RNA was isolated from rat retina, and cDNA was produced and used for PCR cloning to identify the novel subtypes of sPLA(2). Our study revealed the presence of mRNAs encoding sPLA(2)-IB, -X, -V, -IIE, -IIA, and -IIF in the retina, and quantification by real-time PCR revealed different abundances of the sPLA(2)s. We showed a time-dependent gene induction of sPLA(2)-X, -IB, and -V in light-induced retinal damage. We further explored the location of sPLA(2)-IB by in situ hybridization and immunohistochemistry. This study is the first to reveal the presence, abundance, and induction of mRNAs encoding sPLA(2)s in rat retina. We suggest that these enzymes are themselves intercellular signaling modulators of retinal cell function and perhaps also of retinal degeneration.

KW - Animals

KW - Gene Expression Regulation, Enzymologic

KW - Group IB Phospholipases A2

KW - Group II Phospholipases A2

KW - Group X Phospholipases A2

KW - Isoenzymes

KW - Light

KW - Nerve Degeneration

KW - Phospholipases A

KW - Phospholipases A2

KW - RNA, Messenger

KW - Rats

KW - Retina

KW - Retinal Degeneration

KW - Subcellular Fractions

KW - Transcriptional Activation

U2 - 10.1002/jnr.20187

DO - 10.1002/jnr.20187

M3 - Journal article

C2 - 15264221

SN - 0360-4012

VL - 77

SP - 517

EP - 524

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

IS - 4

ER -