TY - JOUR
T1 - Expression and location of mRNAs encoding multiple forms of secretory phospholipase A2 in the rat retina
AU - Kolko, Miriam
AU - Christoffersen, Nanna R
AU - Barreiro, Sebastian G
AU - Bazan, Nicolas G
AU - Kolko, Miriam
N1 - Copyright 2004 Wiley-Liss, Inc.
PY - 2004/8/15
Y1 - 2004/8/15
N2 - Low-molecular-weight secretory phospholipases A(2) (sPLA(2)s) are a subgroup of PLA(2)s, which are secreted, bind to receptors, and may act as intercellular signaling modulators. At least 10 different groups have been characterized in mammals, and there is expanding evidence of the significance of sPLA(2)s in neuronal signaling and survival [Kolko et al. (1996) J. Biol. Chem. 271: 32722-32728]. To date, no retinal sPLA(2)s have been cloned or characterized. We evaluated the existence and abundance of sPLA(2) subtypes in rat retina and explored their possible involvement in light-induced retinal damage. We designed primers to identify the sPLA(2)s in rat retina, based on known sequences of sPLA(2)-specific mRNAs in other tissues. RNA was isolated from rat retina, and cDNA was produced and used for PCR cloning to identify the novel subtypes of sPLA(2). Our study revealed the presence of mRNAs encoding sPLA(2)-IB, -X, -V, -IIE, -IIA, and -IIF in the retina, and quantification by real-time PCR revealed different abundances of the sPLA(2)s. We showed a time-dependent gene induction of sPLA(2)-X, -IB, and -V in light-induced retinal damage. We further explored the location of sPLA(2)-IB by in situ hybridization and immunohistochemistry. This study is the first to reveal the presence, abundance, and induction of mRNAs encoding sPLA(2)s in rat retina. We suggest that these enzymes are themselves intercellular signaling modulators of retinal cell function and perhaps also of retinal degeneration.
AB - Low-molecular-weight secretory phospholipases A(2) (sPLA(2)s) are a subgroup of PLA(2)s, which are secreted, bind to receptors, and may act as intercellular signaling modulators. At least 10 different groups have been characterized in mammals, and there is expanding evidence of the significance of sPLA(2)s in neuronal signaling and survival [Kolko et al. (1996) J. Biol. Chem. 271: 32722-32728]. To date, no retinal sPLA(2)s have been cloned or characterized. We evaluated the existence and abundance of sPLA(2) subtypes in rat retina and explored their possible involvement in light-induced retinal damage. We designed primers to identify the sPLA(2)s in rat retina, based on known sequences of sPLA(2)-specific mRNAs in other tissues. RNA was isolated from rat retina, and cDNA was produced and used for PCR cloning to identify the novel subtypes of sPLA(2). Our study revealed the presence of mRNAs encoding sPLA(2)-IB, -X, -V, -IIE, -IIA, and -IIF in the retina, and quantification by real-time PCR revealed different abundances of the sPLA(2)s. We showed a time-dependent gene induction of sPLA(2)-X, -IB, and -V in light-induced retinal damage. We further explored the location of sPLA(2)-IB by in situ hybridization and immunohistochemistry. This study is the first to reveal the presence, abundance, and induction of mRNAs encoding sPLA(2)s in rat retina. We suggest that these enzymes are themselves intercellular signaling modulators of retinal cell function and perhaps also of retinal degeneration.
KW - Animals
KW - Gene Expression Regulation, Enzymologic
KW - Group IB Phospholipases A2
KW - Group II Phospholipases A2
KW - Group X Phospholipases A2
KW - Isoenzymes
KW - Light
KW - Nerve Degeneration
KW - Phospholipases A
KW - Phospholipases A2
KW - RNA, Messenger
KW - Rats
KW - Retina
KW - Retinal Degeneration
KW - Subcellular Fractions
KW - Transcriptional Activation
U2 - 10.1002/jnr.20187
DO - 10.1002/jnr.20187
M3 - Journal article
C2 - 15264221
SN - 0360-4012
VL - 77
SP - 517
EP - 524
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 4
ER -