Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)

Louise von Gersdorff Jørgensen; Rasmus Demuth Heinecke; Karsten Skjødt; Karina Juhl Rasmussen; Kurt Buchmann

doi:10.1111/j.1365-2761.2011.01291.x

Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)

Louise von Gersdorff Jørgensen, Rasmus Demuth Heinecke, Karsten Skjødt, Karina Juhl Rasmussen, Kurt Buchmann

59 Citations (Scopus)

Abstract

Freshwater fish are able to mount a protective immune response against the parasite Ichthyophthirius multifiliis (Ich) following a non-lethal exposure. Factors involved in immunity comprise cellular and humoral factors, but antibodies have been suggested to play a prominent role in protection. However, host antibodies have not yet been demonstrated to bind to the parasite in situ. By the use of immunohistochemical techniques, this study demonstrated that IgT and IgM bind to surface structures, including cilia, on the early feeding stage of the parasite in the gills of immune rainbow trout, Oncorhynchus mykiss, shortly (2h) after invasion. No binding of IgT and no or only a weak binding of IgM was observed on the parasites in the gills of similarly exposed but naïve rainbow trout. This study indicates that antibodies play an important part in the protection of immune fish against Ich although additional humoral and cellular factors may contribute to this reaction.

Original language	English
Journal	Journal of Fish Diseases
Volume	34
Issue number	10
Pages (from-to)	749-755
Number of pages	7
ISSN	0140-7775
DOIs	https://doi.org/10.1111/j.1365-2761.2011.01291.x
Publication status	Published - Oct 2011

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Jørgensen, L. V. G., Heinecke, R. D., Skjødt, K., Rasmussen, K. J., & Buchmann, K. (2011). Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 34(10), 749-755. https://doi.org/10.1111/j.1365-2761.2011.01291.x

Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum). / Jørgensen, Louise von Gersdorff; Heinecke, Rasmus Demuth; Skjødt, Karsten et al.

In: Journal of Fish Diseases, Vol. 34, No. 10, 10.2011, p. 749-755.

Research output: Contribution to journal › Journal article › Research › peer-review

Jørgensen, LVG, Heinecke, RD, Skjødt, K, Rasmussen, KJ & Buchmann, K 2011, 'Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)', Journal of Fish Diseases, vol. 34, no. 10, pp. 749-755. https://doi.org/10.1111/j.1365-2761.2011.01291.x

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title = "Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)",

abstract = "Freshwater fish are able to mount a protective immune response against the parasite Ichthyophthirius multifiliis (Ich) following a non-lethal exposure. Factors involved in immunity comprise cellular and humoral factors, but antibodies have been suggested to play a prominent role in protection. However, host antibodies have not yet been demonstrated to bind to the parasite in situ. By the use of immunohistochemical techniques, this study demonstrated that IgT and IgM bind to surface structures, including cilia, on the early feeding stage of the parasite in the gills of immune rainbow trout, Oncorhynchus mykiss, shortly (2h) after invasion. No binding of IgT and no or only a weak binding of IgM was observed on the parasites in the gills of similarly exposed but na{\"i}ve rainbow trout. This study indicates that antibodies play an important part in the protection of immune fish against Ich although additional humoral and cellular factors may contribute to this reaction.",

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AU - Heinecke, Rasmus Demuth

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AB - Freshwater fish are able to mount a protective immune response against the parasite Ichthyophthirius multifiliis (Ich) following a non-lethal exposure. Factors involved in immunity comprise cellular and humoral factors, but antibodies have been suggested to play a prominent role in protection. However, host antibodies have not yet been demonstrated to bind to the parasite in situ. By the use of immunohistochemical techniques, this study demonstrated that IgT and IgM bind to surface structures, including cilia, on the early feeding stage of the parasite in the gills of immune rainbow trout, Oncorhynchus mykiss, shortly (2h) after invasion. No binding of IgT and no or only a weak binding of IgM was observed on the parasites in the gills of similarly exposed but naïve rainbow trout. This study indicates that antibodies play an important part in the protection of immune fish against Ich although additional humoral and cellular factors may contribute to this reaction.

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DO - 10.1111/j.1365-2761.2011.01291.x

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Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)

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