Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing libraries

Christel Hougård Enroth; Annaleigh Ohrt Fehler; Line Dahl Poulsen; Jeppe Vinther

doi:10.2144/btn-2018-0178

Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing libraries

Christel Hougård Enroth, Annaleigh Ohrt Fehler, Line Dahl Poulsen, Jeppe Vinther

Computational and RNA Biology

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Original language	English
Journal	BioTechniques
Volume	67
Issue number	3
Pages (from-to)	110-116
ISSN	0736-6205
DOIs	https://doi.org/10.2144/btn-2018-0178
Publication status	Published - 2019

Keywords

cDNA ligation
Exonuclease I
RNA-seq
sequencing library preparation

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10.2144/btn-2018-0178Licence: CC BY-NC-ND

Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing librariesFinal published version, 1.65 MBLicence: CC BY-NC-ND

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@article{54f6c333729341c49eab87615de1107a,

title = "Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing libraries",

keywords = "cDNA ligation, Exonuclease I, RNA-seq, sequencing library preparation",

author = "Enroth, {Christel Houg{\aa}rd} and Fehler, {Annaleigh Ohrt} and Poulsen, {Line Dahl} and Jeppe Vinther",

year = "2019",

doi = "10.2144/btn-2018-0178",

language = "English",

volume = "67",

pages = "110--116",

journal = "BioTechniques",

issn = "0736-6205",

publisher = "Informa Healthcare",

number = "3",

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T1 - Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing libraries

AU - Enroth, Christel Hougård

AU - Fehler, Annaleigh Ohrt

AU - Poulsen, Line Dahl

AU - Vinther, Jeppe

PY - 2019

Y1 - 2019

KW - cDNA ligation

KW - Exonuclease I

KW - RNA-seq

KW - sequencing library preparation

U2 - 10.2144/btn-2018-0178

DO - 10.2144/btn-2018-0178

M3 - Journal article

C2 - 31208218

SN - 0736-6205

VL - 67

SP - 110

EP - 116

JO - BioTechniques

JF - BioTechniques

IS - 3

ER -

Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing libraries

Keywords

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Cite this