Evidence for paracrine/autocrine regulation of GLP-1-producing cells

Camilla Kappe; Qimin Zhang; Jens Juul Holst; Thomas Nyström; Ake Sjöholm

doi:10.1152/ajpcell.00227.2013

Evidence for paracrine/autocrine regulation of GLP-1-producing cells

Camilla Kappe, Qimin Zhang, Jens Juul Holst, Thomas Nyström, Ake Sjöholm

17 Citations (Scopus)

Abstract

Glucagon-like peptide-1 (GLP-1), secreted from gut L cells upon nutrient intake, forms the basis for novel drugs against type 2 diabetes (T2D). Secretion of GLP-1 has been suggested to be impaired in T2D and in conditions associated with hyperlipidemia and insulin resistance. Further, recent studies support lipotoxicity of GLP-1-producing cells in vitro. However, little is known about the regulation of L-cell viability/function, the effects of insulin signaling, or the potential effects of stable GLP-1 analogs and dipeptidyl peptidase-4 (DPP-4) inhibitors. We determined effects of insulin as well as possible autocrine action of GLP-1 on viability/apoptosis of GLP-1-secreting cells in the presence/absence of palmitate, while also assessing direct effects on function. The studies were performed using the GLP-1-secreting cell line GLUTag, and palmitate was used to simulate hyperlipidemia. Our results show that palmitate induced production of reactive oxygen species and caspase-3 activity and reduced cell viability are significantly attenuated by preincubation with insulin/exendin-4. The indicated lipoprotective effect of insulin/exendin-4 was not detectable in the presence of the GLP-1 receptor (GLP-1R) antagonist exendin (9-39) and attenuated in response to pharmacological inhibition of exchange protein activated by cAMP (Epac) signaling, while protein kinase A inhibition had no significant effect. Insulin/exendin-4 also significantly stimulate acute and long-term GLP-1 secretion in the presence of glucose, suggesting novel beneficial effects of insulin signaling and GLP-1R activation on glycemia through enhanced mass of GLP-1-producing cells and enhanced GLP-1 secretion. In addition, the effects of insulin indicate that not only is GLP-1 important for insulin secretion but altered insulin signaling may contribute to an altered GLP-1 secretion.

Original language	English
Journal	A J P: Cell Physiology (Online)
Volume	305
Issue number	10
Pages (from-to)	C1041-9
ISSN	1522-1563
DOIs	https://doi.org/10.1152/ajpcell.00227.2013
Publication status	Published - 15 Nov 2013

Keywords

Animals
Cell Line
Cell Proliferation
Cell Survival
Enteroendocrine Cells
Gene Expression Regulation
Glucagon-Like Peptide 1
Insulin
Mice
Peptides
Reactive Oxygen Species
Receptors, Glucagon
Time Factors
Venoms

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1152/ajpcell.00227.2013

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@article{0001f1ff1a9f46e4b3d7690940586923,

title = "Evidence for paracrine/autocrine regulation of GLP-1-producing cells",

abstract = "Glucagon-like peptide-1 (GLP-1), secreted from gut L cells upon nutrient intake, forms the basis for novel drugs against type 2 diabetes (T2D). Secretion of GLP-1 has been suggested to be impaired in T2D and in conditions associated with hyperlipidemia and insulin resistance. Further, recent studies support lipotoxicity of GLP-1-producing cells in vitro. However, little is known about the regulation of L-cell viability/function, the effects of insulin signaling, or the potential effects of stable GLP-1 analogs and dipeptidyl peptidase-4 (DPP-4) inhibitors. We determined effects of insulin as well as possible autocrine action of GLP-1 on viability/apoptosis of GLP-1-secreting cells in the presence/absence of palmitate, while also assessing direct effects on function. The studies were performed using the GLP-1-secreting cell line GLUTag, and palmitate was used to simulate hyperlipidemia. Our results show that palmitate induced production of reactive oxygen species and caspase-3 activity and reduced cell viability are significantly attenuated by preincubation with insulin/exendin-4. The indicated lipoprotective effect of insulin/exendin-4 was not detectable in the presence of the GLP-1 receptor (GLP-1R) antagonist exendin (9-39) and attenuated in response to pharmacological inhibition of exchange protein activated by cAMP (Epac) signaling, while protein kinase A inhibition had no significant effect. Insulin/exendin-4 also significantly stimulate acute and long-term GLP-1 secretion in the presence of glucose, suggesting novel beneficial effects of insulin signaling and GLP-1R activation on glycemia through enhanced mass of GLP-1-producing cells and enhanced GLP-1 secretion. In addition, the effects of insulin indicate that not only is GLP-1 important for insulin secretion but altered insulin signaling may contribute to an altered GLP-1 secretion.",

keywords = "Animals, Cell Line, Cell Proliferation, Cell Survival, Enteroendocrine Cells, Gene Expression Regulation, Glucagon-Like Peptide 1, Insulin, Mice, Peptides, Reactive Oxygen Species, Receptors, Glucagon, Time Factors, Venoms",

author = "Camilla Kappe and Qimin Zhang and Holst, {Jens Juul} and Thomas Nystr{\"o}m and Ake Sj{\"o}holm",

year = "2013",

month = nov,

day = "15",

doi = "10.1152/ajpcell.00227.2013",

language = "English",

volume = "305",

pages = "C1041--9",

journal = "American Journal of Physiology: Cell Physiology",

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T1 - Evidence for paracrine/autocrine regulation of GLP-1-producing cells

AU - Kappe, Camilla

AU - Zhang, Qimin

AU - Holst, Jens Juul

AU - Nyström, Thomas

AU - Sjöholm, Ake

PY - 2013/11/15

Y1 - 2013/11/15

N2 - Glucagon-like peptide-1 (GLP-1), secreted from gut L cells upon nutrient intake, forms the basis for novel drugs against type 2 diabetes (T2D). Secretion of GLP-1 has been suggested to be impaired in T2D and in conditions associated with hyperlipidemia and insulin resistance. Further, recent studies support lipotoxicity of GLP-1-producing cells in vitro. However, little is known about the regulation of L-cell viability/function, the effects of insulin signaling, or the potential effects of stable GLP-1 analogs and dipeptidyl peptidase-4 (DPP-4) inhibitors. We determined effects of insulin as well as possible autocrine action of GLP-1 on viability/apoptosis of GLP-1-secreting cells in the presence/absence of palmitate, while also assessing direct effects on function. The studies were performed using the GLP-1-secreting cell line GLUTag, and palmitate was used to simulate hyperlipidemia. Our results show that palmitate induced production of reactive oxygen species and caspase-3 activity and reduced cell viability are significantly attenuated by preincubation with insulin/exendin-4. The indicated lipoprotective effect of insulin/exendin-4 was not detectable in the presence of the GLP-1 receptor (GLP-1R) antagonist exendin (9-39) and attenuated in response to pharmacological inhibition of exchange protein activated by cAMP (Epac) signaling, while protein kinase A inhibition had no significant effect. Insulin/exendin-4 also significantly stimulate acute and long-term GLP-1 secretion in the presence of glucose, suggesting novel beneficial effects of insulin signaling and GLP-1R activation on glycemia through enhanced mass of GLP-1-producing cells and enhanced GLP-1 secretion. In addition, the effects of insulin indicate that not only is GLP-1 important for insulin secretion but altered insulin signaling may contribute to an altered GLP-1 secretion.

AB - Glucagon-like peptide-1 (GLP-1), secreted from gut L cells upon nutrient intake, forms the basis for novel drugs against type 2 diabetes (T2D). Secretion of GLP-1 has been suggested to be impaired in T2D and in conditions associated with hyperlipidemia and insulin resistance. Further, recent studies support lipotoxicity of GLP-1-producing cells in vitro. However, little is known about the regulation of L-cell viability/function, the effects of insulin signaling, or the potential effects of stable GLP-1 analogs and dipeptidyl peptidase-4 (DPP-4) inhibitors. We determined effects of insulin as well as possible autocrine action of GLP-1 on viability/apoptosis of GLP-1-secreting cells in the presence/absence of palmitate, while also assessing direct effects on function. The studies were performed using the GLP-1-secreting cell line GLUTag, and palmitate was used to simulate hyperlipidemia. Our results show that palmitate induced production of reactive oxygen species and caspase-3 activity and reduced cell viability are significantly attenuated by preincubation with insulin/exendin-4. The indicated lipoprotective effect of insulin/exendin-4 was not detectable in the presence of the GLP-1 receptor (GLP-1R) antagonist exendin (9-39) and attenuated in response to pharmacological inhibition of exchange protein activated by cAMP (Epac) signaling, while protein kinase A inhibition had no significant effect. Insulin/exendin-4 also significantly stimulate acute and long-term GLP-1 secretion in the presence of glucose, suggesting novel beneficial effects of insulin signaling and GLP-1R activation on glycemia through enhanced mass of GLP-1-producing cells and enhanced GLP-1 secretion. In addition, the effects of insulin indicate that not only is GLP-1 important for insulin secretion but altered insulin signaling may contribute to an altered GLP-1 secretion.

KW - Animals

KW - Cell Line

KW - Cell Proliferation

KW - Cell Survival

KW - Enteroendocrine Cells

KW - Gene Expression Regulation

KW - Glucagon-Like Peptide 1

KW - Insulin

KW - Mice

KW - Peptides

KW - Reactive Oxygen Species

KW - Receptors, Glucagon

KW - Time Factors

KW - Venoms

U2 - 10.1152/ajpcell.00227.2013

DO - 10.1152/ajpcell.00227.2013

M3 - Journal article

C2 - 23986202

SN - 0363-6143

VL - 305

SP - C1041-9

JO - American Journal of Physiology: Cell Physiology

JF - American Journal of Physiology: Cell Physiology

IS - 10

ER -

Evidence for paracrine/autocrine regulation of GLP-1-producing cells

Abstract

Keywords

UN SDGs

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