Abstract
Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.
| Original language | English |
|---|---|
| Journal | Free Radical Research |
| Volume | 43 |
| Issue number | 2 |
| Pages (from-to) | 117-27 |
| Number of pages | 11 |
| ISSN | 1071-5762 |
| DOIs | |
| Publication status | Published - Feb 2009 |
| Externally published | Yes |
Keywords
- Adult
- Blood Proteins
- Blotting, Western
- Case-Control Studies
- Cohort Studies
- Disease Progression
- Electrophoresis, Gel, Two-Dimensional
- Female
- Humans
- Lupus Erythematosus, Systemic
- Male
- Middle Aged
- Oxidation-Reduction
- Oxidative Stress
- Protein Binding
- Severity of Illness Index