Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients

TY - JOUR

T1 - Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients

AU - Morgan, Philip E

AU - Sturgess, Allan D

AU - Davies, Michael Jonathan

PY - 2009/2

Y1 - 2009/2

N2 - Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.

AB - Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.

KW - Adult

KW - Blood Proteins

KW - Blotting, Western

KW - Case-Control Studies

KW - Cohort Studies

KW - Disease Progression

KW - Electrophoresis, Gel, Two-Dimensional

KW - Female

KW - Humans

KW - Lupus Erythematosus, Systemic

KW - Male

KW - Middle Aged

KW - Oxidation-Reduction

KW - Oxidative Stress

KW - Protein Binding

KW - Severity of Illness Index

U2 - 10.1080/10715760802623896

DO - 10.1080/10715760802623896

M3 - Journal article

C2 - 19096973

SN - 1071-5762

VL - 43

SP - 117

EP - 127

JO - Free Radical Research

JF - Free Radical Research

IS - 2

ER -